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Verfasst von:Hammersen, Tim [VerfasserIn]   i
 Buchert, Justyna [VerfasserIn]   i
 Zietzschmann, Severin [VerfasserIn]   i
 Diederichs, Solvig [VerfasserIn]   i
 Richter, Wiltrud [VerfasserIn]   i
Titel:Inverse regulation of cartilage neogenesis at physiologically relevant calcium conditions by human articular chondrocytes and mesenchymal stromal cells
Verf.angabe:Tim Hammersen, Justyna Buchert, Severin Zietzschmann, Solvig Diederichs and Wiltrud Richter
E-Jahr:2023
Jahr:18 June 2023
Umfang:18 S.
Fussnoten:Gesehen am 16.08.2023
Titel Quelle:Enthalten in: Cells
Ort Quelle:Basel : MDPI, 2012
Jahr Quelle:2023
Band/Heft Quelle:12(2023), 12, Artikel-ID 1659, Seite 1-18
ISSN Quelle:2073-4409
Abstract:Elaborate bioreactor cultivation or expensive growth factor supplementation can enhance extracellular matrix production in engineered neocartilage to provide sufficient mechanical resistance. We here investigated whether raising extracellular calcium levels in chondrogenic cultures to physiologically relevant levels would provide a simple and inexpensive alternative to enhance cartilage neogenesis from human articular chondrocytes (AC) or bone marrow-derived mesenchymal stromal cells (BMSC). Interestingly, AC and BMSC-derived chondrocytes showed an opposite response to a calcium increase from 1.8 mM to 8 mM by which glycosaminoglycan (GAG) and collagen type II production were elevated during BMSC chondrogenesis but depressed in AC, leading to two-fold higher GAG/DNA values in BMSC-based neocartilage compared to the AC group. According to control treatments with Mg2+ or sucrose, these effects were specific for CaCl2 rather than divalent cations or osmolarity. Importantly, undesired pro-hypertrophic traits were not stimulated by calcium treatment. Specific induction of PTHrP mRNA and protein by 8.0mM calcium only in AC, along with negative effects of recombinant PTHrP1-34 on cartilage matrix production, suggested that the PTHrP pathway contributed to the detrimental effects in AC-based neocartilage. Altogether, raising extracellular calcium levels was discovered as a novel, simple and inexpensive stimulator for BMSC-based cartilage neogenesis without the need for special bioreactors, whereas such conditions should be avoided for AC.
DOI:doi:10.3390/cells12121659
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext: https://doi.org/10.3390/cells12121659
 Volltext: https://www.mdpi.com/2073-4409/12/12/1659
 DOI: https://doi.org/10.3390/cells12121659
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:chondrogenesis
 extracellular matrix
 parathyroid hormone-related peptide
 stem cells
 tissue engineering
K10plus-PPN:1856321274
Verknüpfungen:→ Zeitschrift

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