Early SARS-CoV-2 infection

• Verfasst von: Rieder, Marina [VerfasserIn]
• Verfasst von: Baldus, Niklas [VerfasserIn]
• Verfasst von: Stallmann, Daniela [VerfasserIn]
• Verfasst von: Jeserich, Maren [VerfasserIn]
• Verfasst von: Goller, Isabella [VerfasserIn]
• Verfasst von: Wirth, Luisa [VerfasserIn]
• Verfasst von: Pollmeier, Luisa [VerfasserIn]
• Verfasst von: Hofmann, Maike [VerfasserIn]
• Verfasst von: Bode, Christoph [VerfasserIn]
• Verfasst von: Busch, Hans-Jörg [VerfasserIn]
• Verfasst von: Schmid, Bonaventura [VerfasserIn]
• Verfasst von: Gauchel, Nadine [VerfasserIn]
• Verfasst von: Scharf, Rüdiger Eberhard [VerfasserIn]
• Verfasst von: Dürschmied, Daniel [VerfasserIn]
• Verfasst von: Lother, Achim [VerfasserIn]
• Verfasst von: Krauel, Krystin [VerfasserIn]
• Titel: Early SARS-CoV-2 infection
• Titelzusatz: platelet-neutrophil complexes and platelet function
• Verf.angabe: Marina Rieder, Niklas Baldus, Daniela Stallmann, Maren Jeserich, Isabella Goller, Luisa Wirth, Luisa Pollmeier, Maike Hofmann, Christoph Bode, Hans-Joerg Busch, Bonaventura Schmid, Nadine Gauchel, Rüdiger E. Scharf, Daniel Duerschmied, Achim Lother, Krystin Krauel
• E-Jahr: 2023
• Jahr: January 2023
• Umfang: 12 S.
• Illustrationen: Illustrationen
• Fussnoten: Online verfügbar: 23. Dezember 2022, Artikelversion: 11. März 2023 ; Gesehen am 22.08.2023
• Titel Quelle: Enthalten in: Research and practice in thrombosis and haemostasis
• Ort Quelle: [Amsterdam] : Elsevier, 2017
• Jahr Quelle: 2023
• Band/Heft Quelle: 7(2023), 1 vom: Jan., Artikel-ID 100025, Seite 1-12
• ISSN Quelle: 2475-0379
• DOI: doi:10.1016/j.rpth.2022.100025
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: COVID-19
• Sach-SW: neutrophils
• Sach-SW: platelet aggregation
• Sach-SW: platelets
• Sach-SW: SARS-CoV-2
• K10plus-PPN: 1857214765

Abstract

Background - Conflicting results have been reported on platelet activity ex vivo and responsiveness in vitro among patients with COVID-19 with or without thromboembolic complications. - Objectives - To assess platelet reactivity in patients with moderate disease at early stages of COVID-19. - Methods - We performed a prospective, descriptive analysis of 100 consecutive patients presenting with suspected SARS-CoV-2 infection at University Medical Center Freiburg during the first or second wave of the pandemic. Following polymerase chain reaction testing and compliance with study inclusion criteria, 20 SARS-CoV-2-positive and 55 SARS-CoV-2-negative patients (serving as patient controls) were enrolled. In addition, 15 healthy subjects were included. Platelet reactivity was assessed using whole-blood impedance aggregometry and flow cytometry in response to various agonists. - Results - Platelet aggregation was significantly impaired in the patients with COVID-19 compared with that in the patient controls or healthy subjects. The reduced platelet responsiveness in the patients with COVID-19 was associated with impaired activation of GPIIb/IIIa (αIIbβ3). In contrast, low expression of P-selectin at baseline and intact secretion upon stimulation in vitro suggest that no preactivation in vivo, leading to “exhausted” platelets, had occurred. The proportion of circulating platelet-neutrophil complexes was significantly higher in the patients with COVID-19 (mean ± SD, 41% ± 13%) than in the patient controls (18% ± 7%; 95% CI, 11.1-34.1; P = .0002) or healthy subjects (17% ± 4%; 95% CI, 13.8-33.8; P < .0001). An analysis of neutrophil adhesion receptors revealed upregulation of CD11b (α-subunit of αMβ2) and CD66b (CEACAM8) but not of CD162 (PSGL-1) in the patients with COVID-19. - Conclusion - Despite reduced platelet responsiveness, platelet-neutrophil complexes are increased at early stages of moderate disease. Thus, this cellular interaction may occur during COVID-19 without preceding platelet activation.