Force-induced destabilization of focal adhesions at defined integrin spacings on nanostructured surfaces

• Verfasst von: Beer, Alex G. F. de [VerfasserIn]
• Verfasst von: Cavalcanti-Adam, Elisabetta A. [VerfasserIn]
• Verfasst von: Majer, Günter [VerfasserIn]
• Verfasst von: Lopez-García, M. [VerfasserIn]
• Verfasst von: Kessler, H. [VerfasserIn]
• Verfasst von: Spatz, Joachim P. [VerfasserIn]
• Titel: Force-induced destabilization of focal adhesions at defined integrin spacings on nanostructured surfaces
• Verf.angabe: Alex G.F. de Beer, E. Ada Cavalcanti-Adam, Günter Majer, M. Lopez-García, H. Kessler, and Joachim P. Spatz
• E-Jahr: 2010
• Jahr: 12 May 2010
• Umfang: 7 S.
• Illustrationen: Illustrationen
• Fussnoten: Gesehen am 04.09.2023
• Titel Quelle: Enthalten in: Physical review / E
• Ort Quelle: College Park, Md. : APS, 1993
• Jahr Quelle: 2010
• Band/Heft Quelle: 81(2010), 5 vom: Mai, Artikel-ID 051914, Seite 1-7
• ISSN Quelle: 1550-2376
• DOI: doi:10.1103/PhysRevE.81.051914
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1858666511

Abstract

Focal adhesions are the anchoring points of cells to surfaces and are responsible for a large number of surface sensing processes. Nanopatterning studies have shown physiological changes in fibroblasts as a result of decreasing density of external binding ligands. The most striking of these changes is a decreased ability to form mature focal adhesions when lateral ligand distances exceed 76 nm. These changes are usually examined in the context of protein signaling and protein interactions. We show a physical explanation based on the balance between the forces acting on individual ligand connections and the reaction kinetics of those ligands. We propose three stability regimes for focal adhesions as a function of ligand spacing and applied stress: a stable regime, an unstable regime in which a large fraction of unbound protein causes adhesion disintegration, and a regime in which the applied force is too high to form an adhesion structure.