| |  Online-Ressource |
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| Verfasst von: | Pichler, Andrea [VerfasserIn]  |
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| | Knipscheer, Puck [VerfasserIn] |
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| | Oberhofer, Edith [VerfasserIn] |
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| | van Dijk, Willem J. [VerfasserIn] |
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| | Körner, Roman [VerfasserIn] |
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| | Olsen, Jesper Velgaard [VerfasserIn] |
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| | Jentsch, Stefan [VerfasserIn] |
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| | Melchior, Frauke [VerfasserIn] |
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| | Sixma, Titia K. [VerfasserIn] |
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| Titel: | SUMO modification of the ubiquitin-conjugating enzyme E2-25K |
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| Verf.angabe: | Andrea Pichler, Puck Knipscheer, Edith Oberhofer, Willem J. van Dijk, Roman Körner, Jesper Velgaard Olsen, Stefan Jentsch, Frauke Melchior & Titia K. Sixma |
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| E-Jahr: | 2005 |
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| Jahr: | 20 February 2005 |
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| Umfang: | 6 S. |
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| Fussnoten: | Gesehen am 04.09.2023 |
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| Titel Quelle: | Enthalten in: Nature structural & molecular biology |
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| Ort Quelle: | London [u.a.] : Nature Publishing Group, 2004 |
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| Jahr Quelle: | 2005 |
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| Band/Heft Quelle: | 12(2005), 3, Seite 264-269 |
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| ISSN Quelle: | 1545-9985 |
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| Abstract: | Post-translational modification with small ubiquitin-related modifier (SUMO) alters the function of many proteins, but the molecular mechanisms and consequences of this modification are still poorly defined. During a screen for novel SUMO1 targets, we identified the ubiquitin-conjugating enzyme E2-25K (Hip2). SUMO attachment severely impairs E2-25K ubiquitin thioester and unanchored ubiquitin chain formation in vitro. Crystal structures of E2-25K(1-155) and of the E2-25K(1-155)-SUMO conjugate (E2-25K*SUMO) indicate that SUMO attachment interferes with E1 interaction through its location on the N-terminal helix. The SUMO acceptor site in E2-25K, Lys14, does not conform to the consensus site found in most SUMO targets (ΨKXE), and functions only in the context of an α-helix. In contrast, adjacent SUMO consensus sites are modified only when in unstructured peptides. The demonstration that secondary structure elements are part of SUMO attachment signals could contribute to a better prediction of SUMO targets. |
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| DOI: | doi:10.1038/nsmb903 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1038/nsmb903 |
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| | Volltext: https://www.nature.com/articles/nsmb903 |
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| | DOI: https://doi.org/10.1038/nsmb903 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | Biochemistry |
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| | Biological Microscopy |
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| | general |
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| | Life Sciences |
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| | Membrane Biology |
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| | Protein Structure |
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| K10plus-PPN: | 1858760682 |
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| Verknüpfungen: | → Zeitschrift |
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SUMO modification of the ubiquitin-conjugating enzyme E2-25K / Pichler, Andrea [VerfasserIn]; 20 February 2005 (Online-Ressource)
