Impact of salt intake and renin-angiotensin-aldosterone system blockade on lung severe acute respiratory syndrome coronavirus 2 host factors

• Verfasst von: Zhang, Xiaoli [VerfasserIn]
• Verfasst von: Liu, Liping [VerfasserIn]
• Verfasst von: Gaballa, Mohamed Mahmoud Salem Ahmed [VerfasserIn]
• Verfasst von: Hasan, Ahmed A. [VerfasserIn]
• Verfasst von: Xiong, Yingquan [VerfasserIn]
• Verfasst von: Xie, Li [VerfasserIn]
• Verfasst von: Klein, Thomas [VerfasserIn]
• Verfasst von: Delic, Denis [VerfasserIn]
• Verfasst von: Kleuser, Burkhard [VerfasserIn]
• Verfasst von: Krämer, Bernhard [VerfasserIn]
• Verfasst von: Li, Jian [VerfasserIn]
• Verfasst von: Hocher, Berthold [VerfasserIn]
• Titel: Impact of salt intake and renin-angiotensin-aldosterone system blockade on lung severe acute respiratory syndrome coronavirus 2 host factors
• Verf.angabe: Xiaoli Zhang, Liping Liu, Mohamed M.S. Gaballa, Ahmed A. Hasan, Yingquan Xiong, Li Xie, Thomas Klein, Denis Delic, Burkhard Kleuser, Bernhard K. Krämer, Jian Li, Berthold Hocher
• E-Jahr: 2022
• Jahr: July 25, 2022
• Umfang: 11 S.
• Illustrationen: Illustrationen
• Fussnoten: Gesehen am 12.09.2023
• Titel Quelle: Enthalten in: Kidney & blood pressure research
• Ort Quelle: Basel [u.a.] : Karger, 1996
• Jahr Quelle: 2022
• Band/Heft Quelle: 47(2022), 9 vom: Okt., Seite 565-575
• ISSN Quelle: 1423-0143
• DOI: doi:10.1159/000525368
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1859357547

Abstract

Introduction: The angiotensin-converting enzyme 2 (ACE2) as well as the transmembrane protease serine type 2 (TMPRSS2) have been found to play roles in cell entry for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the virus causing coronavirus disease 2019 (COVID-19). SARS-CoV-2 infection risk and severity of COVID-19 might be indicated by the expression of ACE2 and TMPRSS2 in the lung. Methods: A high-salt diet rat model and renin-angiotensin-aldosterone system (RAAS) blockade were used to test whether these factors affect ACE2 and TMPRSS2 expression in the lung. A normal (0.3% NaCl), a medium (2% NaCl), or a high (8% NaCl) salt diet was fed to rats for 12 weeks, along with enalapril or telmisartan, before examining the lung for histopathological alteration. Using immunofluorescence and qRT-PCR, the localization as well as mRNA expression of ACE2 and TMPRSS2 were investigated. Results: The findings provide evidence that both TMPRSS2 and ACE2 are highly expressed in bronchial epithelial cells as well as ACE2 was also expressed in alveolar type 2 cells. High-salt diet exposure in rats leads to elevated ACE2 expression on protein level. Treatment with RAAS blockers had no effect on lung tissue expression of ACE2 and TMPRSS2. Conclusions: These findings offer biological support regarding the safety of these drugs that are often prescribed to COVID-19 patients with cardiovascular comorbidity. High salt intake, on the other hand, might adversely affect COVID-19 outcome. Our preclinical data should stimulate clinical studies addressing this point of concern.