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Verfasst von:An, Liying [VerfasserIn]   i
 Michaeli, Julia [VerfasserIn]   i
 Pallavi, Prama [VerfasserIn]   i
 Breedijk, Annette [VerfasserIn]   i
 Xu, Xin [VerfasserIn]   i
 Dietrich, Nadine [VerfasserIn]   i
 Sigl, Martin [VerfasserIn]   i
 Keese, Michael [VerfasserIn]   i
 Nitschke, Katja [VerfasserIn]   i
 Jarczyk, Jonas [VerfasserIn]   i
 Nuhn, Philipp [VerfasserIn]   i
 Krämer, Bernhard [VerfasserIn]   i
 Yard, Benito A. [VerfasserIn]   i
 Leipe, Jan [VerfasserIn]   i
Titel:Concurrent stimulation of monocytes with CSF1 and polarizing cytokines reveals phenotypic and functional differences with classical polarized macrophages
Verf.angabe:Liying An, Julia Michaeli, Prama Pallavi, Annette Breedijk, Xin Xu, Nadine Dietrich, Martin Sigl, Michael Keese, Katja Nitschke, Jonas Jarczyk, Philipp Nuhn, Bernhard K Krämer, Benito A Yard, Jan Leipe
E-Jahr:2022
Jahr:24 January 2022
Umfang:11 S.
Fussnoten:Gesehen am 18.09.2023
Titel Quelle:Enthalten in: Journal of leukocyte biology
Ort Quelle:Kettering : Oxford University Press, 1984
Jahr Quelle:2022
Band/Heft Quelle:112(2022), 3 vom: Sept., Seite 437-447
ISSN Quelle:1938-3673
Abstract:In atherosclerotic lesions, macrophages are exposed to CSFs and various microenvironmental cues, which ultimately drive their polarization state. We studied the expression of different CSFs in artery specimen and cultured vascular cells and assessed whether concurrent stimulation (CS) of monocytes with CSF1 and polarizing cytokines generated macrophages (CSM1 and CSM2) that were phenotypically and functionally different from classically polarized M1 and M2 macrophages. We also assessed the influence of acetylsalicylic acid (ASA) on the capacity of polarized macrophages to stimulate T-cell proliferation. CSF1 was the most prominent CSF expressed in arteries and cultured vascular cells. M1 and CSM1 macrophages differed in CD86 and CD14 expression, which was up-regulated respectively down-regulated by LPS. M2 and CSM2 macrophages were phenotypically similar. Cyclooxygenase expression was different in CSM1 (COX-1− and COX-2+ after LPS stimulation) and CSM2 (COX-1+ and COX-2−) macrophages. TNFα production was more pronounced in CSM1 macrophages, whereas IL-10 was produced at higher levels by CSM2 macrophages. Proliferation of allogeneic T cells was strongly supported by CSM2, but not by CSM1 polarized macrophages. Although ASA did not affect anti-CD3/CD28-mediated proliferation, it significantly reduced CSM2 and CSM1-mediated T-cell proliferation. Supernatants of LPS-stimulated CSM2 but not of CSM1 macrophages could overcome the inhibition by ASA. Hence, we demonstrate that CSM1 and CSM2 macrophages are phenotypically and to some extent functionally distinct from classically polarized M1 and M2 macrophages. CSM2 macrophages produce a COX-1-dependent soluble factor that supports T-cell proliferation, the identity hereof is still elusive and warrants further studies.
DOI:doi:10.1002/JLB.3A0721-383R
URL:kostenfrei: Volltext: https://doi.org/10.1002/JLB.3A0721-383R
 kostenfrei: Volltext: https://academic.oup.com/jleukbio/article/112/3/437/6976174?login=true
 DOI: https://doi.org/10.1002/JLB.3A0721-383R
Datenträger:Online-Ressource
Sprache:eng
K10plus-PPN:1859796214
Verknüpfungen:→ Zeitschrift
 
 
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