| |  Online-Ressource |
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| Verfasst von: | Voigt, Jonah L. [VerfasserIn]  |
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| | Timmer, Jens [VerfasserIn] |
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| | Pennarola, Federica [VerfasserIn] |
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| | Christian, Joel [VerfasserIn] |
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| | Meng, Ning [VerfasserIn] |
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| | Blumberg, Johannes [VerfasserIn] |
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| | Schwarz, Ulrich S. [VerfasserIn] |
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| | Grimm, Dirk [VerfasserIn] |
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| | Cavalcanti-Adam, Elisabetta A. [VerfasserIn] |
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| Titel: | Substrate stiffness and particle properties influence cellular uptake of nanoparticles and viruses from the ventral side |
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| Verf.angabe: | Jonah L. Voigt, Jens Timmer, Federica Pennarola, Joel Christian, Ning Meng, Johannes W. Blumberg, Ulrich S. Schwarz, Dirk Grimm, and Elisabetta Ada Cavalcanti-Adam |
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| Jahr: | 2024 |
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| Umfang: | Illustrationen, Diagramme |
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| | 13 S. |
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| Fussnoten: | Gesehen am 19.09.2023 ; Zuerst veröffentlicht: 23. August 2023 |
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| Titel Quelle: | Enthalten in: Advanced functional materials |
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| Ort Quelle: | Weinheim : Wiley-VCH, 2001 |
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| Jahr Quelle: | 2024 |
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| Band/Heft Quelle: | 34(2024), 35, Artikel-ID 2304674, Seite 1-13 |
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| ISSN Quelle: | 1616-3028 |
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| Abstract: | It is a long-standing challenge to exploit cellular uptake mechanisms to deliver desired cargo into cells, for example, specific drugs or gene editing techniques. This study introduces a bioinspired material approach where nanoparticles are presented at the ventral side of cells adhering to engineered extracellular matrices. The effect of matrix stiffness on cell adhesion and mechanics, as well as on particle internalization by clathrin-mediated endocytosis (CME), is investigated for varying particle size and surface functionalization. The results presented here show that substrate stiffness affects both cell adhesion and particle internalization, with softer substrates promoting higher levels of particle uptake. However, the activation of the CME pathway, either mechanically by particle size or functionally by receptor binding, regulates the sensitivity of cellular particle uptake to matrix stiffness. Finally, adeno-associated viruses as the leading platform for therapeutic gene delivery are used as model cargo to showcase the importance of considering multiple components when designing delivery systems. These findings indicate that particle uptake is a multifaceted process that can be improved by the appropriate combination of extracellular environment mechanics and cargo properties. |
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| DOI: | doi:10.1002/adfm.202304674 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
kostenfrei: Volltext: https://doi.org/10.1002/adfm.202304674 |
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| | kostenfrei: Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1002/adfm.202304674 |
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| | DOI: https://doi.org/10.1002/adfm.202304674 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | actomyosin contractility |
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| | adeno-associated virus |
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| | cellular uptake |
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| | clathrin-mediated endocytosis |
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| | mechanotargeting |
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| | nanoparticles |
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| | substrate stiffness |
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| K10plus-PPN: | 1859875815 |
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| Verknüpfungen: | → Zeitschrift |
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Substrate stiffness and particle properties influence cellular uptake of nanoparticles and viruses from the ventral side / Voigt, Jonah L. [VerfasserIn]; 2024 (Online-Ressource)
