| Online-Ressource |
Verfasst von: | Wabnitz, Guido H. [VerfasserIn]  |
| Lohneis, Philipp [VerfasserIn]  |
| Kirchgessner, Henning [VerfasserIn]  |
| Jahraus, Beate [VerfasserIn]  |
| Gottwald, Susan [VerfasserIn]  |
| Konstandin, Mathias [VerfasserIn]  |
| Klemke, Martin [VerfasserIn]  |
| Samstag, Yvonne [VerfasserIn]  |
Titel: | Sustained LFA-1 cluster formation in the immune synapse requires the combined activities of L-plastin and calmodulin |
Verf.angabe: | Guido H. Wabnitz, Philipp Lohneis, Henning Kirchgessner, Beate Jahraus, Susan Gottwald, Mathias Konstandin, Martin Klemke and Yvonne Samstag |
E-Jahr: | 2010 |
Jahr: | 26 August 2010 |
Umfang: | 13 S. |
Illustrationen: | Illustrationen |
Fussnoten: | Gesehen am 19.09.2023 |
Titel Quelle: | Enthalten in: European journal of immunology |
Ort Quelle: | Weinheim : Wiley-VCH, 1971 |
Jahr Quelle: | 2010 |
Band/Heft Quelle: | 40(2010), 9 vom: Sept., Seite 2437-2449 |
ISSN Quelle: | 1521-4141 |
Abstract: | Formation of immune synapses (IS) between T cells and APC requires multiple rearrangements in the actin cytoskeleton and selective receptor accumulation in supramolecular activation clusters (SMAC). The inner cluster (central SMAC) contains the TCR/CD3 complex. The outer cluster (peripheral SMAC) contains the integrin LFA-1 and Talin. Molecular mechanisms selectively stabilizing receptors in the IS remained largely unknown. Here, we demonstrate that sustained LFA-1 clustering in the IS is a consequence of the combined activities of the actin-bundling protein L-plastin (LPL) and calmodulin. Thus, upon antigen-recognition of T cells, LPL accumulated predominantly in the peripheral SMAC. siRNA-mediated knock-down of LPL led to a failure of LFA-1 and Talin redistribution - however, not TCR/CD3 relocalization - into the IS. As a result of this LPL knock-down, the T-cell/APC interface became smaller over time and T-cell proliferation was inhibited. Importantly, binding of calmodulin to LPL was required for the maintenance of LPL in the IS and consequently inhibition of calmodulin also prevented stable accumulation of LFA-1 and Talin, but not CD3, in the IS. |
DOI: | doi:10.1002/eji.201040345 |
URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
kostenfrei: Volltext: https://doi.org/10.1002/eji.201040345 |
| kostenfrei: Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1002/eji.201040345 |
| DOI: https://doi.org/10.1002/eji.201040345 |
Datenträger: | Online-Ressource |
Sprache: | eng |
Sach-SW: | Actin cytoskeleton |
| Immunological synapse |
| T cells |
K10plus-PPN: | 1859932649 |
Verknüpfungen: | → Zeitschrift |
Sustained LFA-1 cluster formation in the immune synapse requires the combined activities of L-plastin and calmodulin / Wabnitz, Guido H. [VerfasserIn]; 26 August 2010 (Online-Ressource)