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Status: Bibliographieeintrag

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Verfasst von:Li, Hui [VerfasserIn]   i
 Breedijk, Annette [VerfasserIn]   i
 Dietrich, Nadine [VerfasserIn]   i
 Nitschke, Katja [VerfasserIn]   i
 Jarczyk, Jonas [VerfasserIn]   i
 Nuhn, Philipp [VerfasserIn]   i
 Krämer, Bernhard [VerfasserIn]   i
 Yard, Benito A. [VerfasserIn]   i
 Leipe, Jan [VerfasserIn]   i
 Hauske, Sibylle J. [VerfasserIn]   i
Titel:Lipopolysaccharide tolerance in human primary monocytes and polarized macrophages
Verf.angabe:Hui Li, Annette Breedijk, Nadine Dietrich, Katja Nitschke, Jonas Jarczyk, Philipp Nuhn, Bernhard K. Krämer, Benito A. Yard, Jan Leipe and Sibylle Hauske
Jahr:2023
Umfang:17 S.
Illustrationen:Illustrationen
Fussnoten:Veröffentlicht: 30. Juli 2023 ; Gesehen am 21.09.2023
Titel Quelle:Enthalten in: International journal of molecular sciences
Ort Quelle:Basel : Molecular Diversity Preservation International, 2000
Jahr Quelle:2023
Band/Heft Quelle:24(2023), 15, Artikel-ID 12196, Seite 1-17
ISSN Quelle:1422-0067
 1661-6596
Abstract:Innate immune memory allows macrophages to adequately respond to pathogens to which they have been pre-exposed. To what extent different pattern recognition receptors, cytokines and resolution signals influence innate immune memory needs further elucidation. The present study assessed whether lipopolysaccharide (LPS) tolerance in monocytes and macrophages is affected by these factors. Human CD14+ cells were isolated from peripheral blood, stimulated by LPS and re-stimulated after 3 days of resting. Hereafter, immune-responsive gene 1 (IRG-1), heme oxygenase 1 (HO-1), tumor necrosis factor α (TNF-α) and interleukin 6 (IL-6) expression were assessed. Our study revealed the following findings: (1) While pre-stimulation with the Toll-like receptor 4 ligand LPS inhibits the induction of IRG-1, TNF-α and IL-6 expression, pre-stimulation with TLR 1/2 ligands only affects cytokine production but not IRG-1 expression upon subsequent TLR4 engagement. (2) Prior TNF-α stimulation does not affect LPS tolerance but rather increases LPS-mediated cytokine expression. (3) Dimethyl itaconate (DMI) inhibits the expression of IRG-1 in a dose-dependent manner but does not affect TNF-α or IL-6 expression. (4) Docosahexaenoic acid (DHA) partly inhibits IRG-1 expression in monocytes but not in M(IFNγ) and M(IL-4) polarized macrophages. LPS tolerance is not affected in these cells by DHA. The data presented in this study partly corroborate and extend previous findings on innate immune memory and warrant further studies on LPS tolerance to gain a better understanding of innate immune memory at the molecular level.
DOI:doi:10.3390/ijms241512196
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

kostenfrei: Volltext: https://doi.org/10.3390/ijms241512196
 kostenfrei: Volltext: https://www.mdpi.com/1422-0067/24/15/12196
 DOI: https://doi.org/10.3390/ijms241512196
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:DHA
 DMI
 innate immune memory
 LPS tolerance
 macrophages
 monocytes
 TLR agonists
 trained immunity
K10plus-PPN:1860160514
Verknüpfungen:→ Zeitschrift

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