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| Verfasst von: | Schreiber, Pamela [VerfasserIn]  |
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| | Friedrich, Ann-Kathrin [VerfasserIn] |
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| | Gruber, Gefion [VerfasserIn] |
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| | Nußhag, Christian [VerfasserIn] |
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| | Boegelein, Lukas [VerfasserIn] |
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| | Eßbauer, Sandra [VerfasserIn] |
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| | Uhrig, Josephine [VerfasserIn] |
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| | Zeier, Martin [VerfasserIn] |
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| | Krautkrämer, Ellen [VerfasserIn] |
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| Titel: | Differences in the susceptibility of human tubular epithelial cells for infection with orthohantaviruses |
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| Verf.angabe: | Pamela Schreiber, Ann-Kathrin Friedrich, Gefion Gruber, Christian Nusshag, Lukas Boegelein, Sandra Essbauer, Josephine Uhrig, Martin Zeier, Ellen Krautkrämer |
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| E-Jahr: | 2023 |
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| Jahr: | 31 July 2023 |
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| Umfang: | 13 S. |
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| Fussnoten: | Gesehen am 16.10.2023 |
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| Titel Quelle: | Enthalten in: Viruses |
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| Ort Quelle: | Basel : MDPI, 2009 |
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| Jahr Quelle: | 2023 |
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| Band/Heft Quelle: | 15(2023), 8, Artikel-ID 1670, Seite 1-13 |
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| ISSN Quelle: | 1999-4915 |
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| Abstract: | Diseases induced by infection with pathogenic orthohantaviruses are characterized by a pronounced organ-specific manifestation. Pathogenic Eurasian orthohantaviruses cause hemorrhagic fever with renal syndrome (HFRS) with often massive proteinuria. Therefore, the use of a relevant kidney cell culture would be favorable to analyze the underlying cellular mechanisms of orthohantavirus-induced acute kidney injury (AKI). We tested different human tubular epithelial cell lines for their suitability as an in vitro infection model. Permissiveness and replication kinetics of highly pathogenic Hantaan virus (HTNV) and non-/low-pathogenic Tula virus (TULV) were analyzed in tubular epithelial cell lines and compared to human primary tubular epithelial cells. Ana-lysis of the cell line HK-2 revealed the same results for viral replication, morphological and functional effects as observed for HTNV in primary cells. In contrast, the cell lines RPTEC/TERT1 and TH1 demonstrated only poor infection rates after inoculation with HTNV and are unusable as an infection model. While pathogenic HNTV infects primary tubular and HK-2 cells, non-/low-pathogenic TULV infects neither primary tubular cells nor the cell line HK-2. Our results show that permissiveness of renal cells varies between orthohantaviruses with differences in pathogenicity and that HK-2 cells demonstrate a suitable in vitro model to study viral tropism and pathogenesis of orthohantavirus-induced AKI. |
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| DOI: | doi:10.3390/v15081670 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
kostenfrei: Volltext: https://doi.org/10.3390/v15081670 |
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| | kostenfrei: Volltext: https://www.mdpi.com/1999-4915/15/8/1670 |
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| | DOI: https://doi.org/10.3390/v15081670 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | acute kidney injury |
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| | cell-to-cell contacts |
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| | Hantaan virus |
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| | kidney |
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| | orthohantavirus |
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| | pathogenesis |
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| | receptor |
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| | tubular epithelium |
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| | Tula virus |
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| K10plus-PPN: | 1865766526 |
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| Verknüpfungen: | → Zeitschrift |
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Differences in the susceptibility of human tubular epithelial cells for infection with orthohantaviruses / Schreiber, Pamela [VerfasserIn]; 31 July 2023 (Online-Ressource)
