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| Verfasst von: | Bendig, Tamara [VerfasserIn]  |
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| | Ulmer, Andreas [VerfasserIn] |
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| | Luzia, Laura [VerfasserIn] |
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| | Müller, Susanne [VerfasserIn] |
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| | Sahle, Sven [VerfasserIn] |
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| | Bergmann, Frank T. [VerfasserIn] |
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| | Lösch, Maren [VerfasserIn] |
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| | Erdemann, Florian [VerfasserIn] |
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| | Zeidan, Ahmad A. [VerfasserIn] |
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| | Mendoza, Sebastian N. [VerfasserIn] |
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| | Teusink, Bas [VerfasserIn] |
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| | Takors, Ralf [VerfasserIn] |
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| | Kummer, Ursula [VerfasserIn] |
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| | Figueiredo, Ana Sofia [VerfasserIn] |
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| Titel: | The pH-dependent lactose metabolism of Lactobacillus delbrueckii subsp. bulgaricus |
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| Titelzusatz: | an integrative view through a mechanistic computational model |
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| Verf.angabe: | Tamara Bendig, Andreas Ulmer, Laura Luzia, Susanne Müller, Sven Sahle, Frank T. Bergmann, Maren Lösch, Florian Erdemann, Ahmad A. Zeidan, Sebastian N. Mendoza, Bas Teusink, Ralf Takors, Ursula Kummer, Ana Sofia Figueiredo |
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| E-Jahr: | 2023 |
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| Jahr: | 10 September 2023 |
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| Umfang: | 11 S. |
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| Illustrationen: | Illustrationen |
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| Fussnoten: | Online verfügbar: 10. August 2023, Artikelversion: 18. August 2023 ; Gesehen am 18.10.2023 |
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| Titel Quelle: | Enthalten in: Journal of biotechnology |
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| Ort Quelle: | Amsterdam [u.a.] : Elsevier Science, 1984 |
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| Jahr Quelle: | 2023 |
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| Band/Heft Quelle: | 374(2023) vom: Aug., Seite 90-100 |
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| ISSN Quelle: | 1873-4863 |
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| Abstract: | The fermentation process of milk to yoghurt using Lactobacillus delbrueckii subsp. bulgaricus in co-culture with Streptococcus thermophilus is hallmarked by the breakdown of lactose to organic acids such as lactate. This leads to a substantial decrease in pH - both in the medium, as well as cytosolic. The latter impairs metabolic activities due to the pH-dependence of enzymes, which compromises microbial growth. To quantitatively elucidate the impact of the acidification on metabolism of L. bulgaricus in an integrated way, we have developed a proton-dependent computational model of lactose metabolism and casein degradation based on experimental data. The model accounts for the influence of pH on enzyme activities as well as cellular growth and proliferation of the bacterial population. We used a machine learning approach to quantify the cell volume throughout fermentation. Simulation results show a decrease in metabolic flux with acidification of the cytosol. Additionally, the validated model predicts a similar metabolic behaviour within a wide range of non-limiting substrate concentrations. This computational model provides a deeper understanding of the intricate relationships between metabolic activity and acidification and paves the way for further optimization of yoghurt production under industrial settings. |
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| DOI: | doi:10.1016/j.jbiotec.2023.08.001 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
kostenfrei: Volltext: https://doi.org/10.1016/j.jbiotec.2023.08.001 |
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| | kostenfrei: Volltext: https://www.sciencedirect.com/science/article/pii/S0168165623001414 |
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| | DOI: https://doi.org/10.1016/j.jbiotec.2023.08.001 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | Cytosolic pH |
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| | Intracellular pH |
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| | Kinetic model |
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| | Lactic acid bacteria |
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| | Lactose metabolism |
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| K10plus-PPN: | 186620128X |
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| Verknüpfungen: | → Zeitschrift |
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¬The¬ pH-dependent lactose metabolism of Lactobacillus delbrueckii subsp. bulgaricus / Bendig, Tamara [VerfasserIn]; 10 September 2023 (Online-Ressource)
