Transcription activator WCC recruits deacetylase HDA3 to control transcription dynamics and bursting in Neurospora

• Verfasst von: Oehler, Michael [VerfasserIn]
• Verfasst von: Geisser, Leonie [VerfasserIn]
• Verfasst von: Diernfellner, Axel [VerfasserIn]
• Verfasst von: Brunner, Michael [VerfasserIn]
• Titel: Transcription activator WCC recruits deacetylase HDA3 to control transcription dynamics and bursting in Neurospora
• Verf.angabe: Michael Oehler, Leonie Geisser, Axel C.R. Diernfellner, Michael Brunner
• E-Jahr: 2023
• Jahr: Jun 2023
• Umfang: 13 S.
• Fussnoten: Online: 30. Juni 2023 ; Gesehen am 23.10.2023
• Titel Quelle: Enthalten in: Science advances
• Ort Quelle: Washington, DC [u.a.] : Assoc., 2015
• Jahr Quelle: 2023
• Band/Heft Quelle: 9(2023), 26, Artikel-ID eadh0721, Seite 1-13
• ISSN Quelle: 2375-2548
• DOI: doi:10.1126/sciadv.adh0721
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1867279088

Abstract

RNA polymerase II initiates transcription either randomly or in bursts. We examined the light-dependent transcriptional activator White Collar Complex (WCC) of Neurospora to characterize the transcriptional dynamics of the strong vivid (vvd) promoter and the weaker frequency (frq) promoter. We show that WCC is not only an activator but also represses transcription by recruiting histone deacetylase 3 (HDA3). Our data suggest that bursts of frq transcription are governed by a long-lived refractory state established and maintained by WCC and HDA3 at the core promoter, whereas transcription of vvd is determined by WCC binding dynamics at an upstream activating sequence. Thus, in addition to stochastic binding of transcription factors, transcription factor-mediated repression may also influence transcriptional bursting.