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Status: Bibliographieeintrag

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Verfasst von:Huck, Volker [VerfasserIn]   i
 Chen, Po-Chia [VerfasserIn]   i
 Xu, Emma-Ruoqi [VerfasserIn]   i
 Tischer, Alexander [VerfasserIn]   i
 Klemm, Ulrike [VerfasserIn]   i
 Aponte-Santamaría, Camilo [VerfasserIn]   i
 Meß, Christian [VerfasserIn]   i
 Obser, Tobias [VerfasserIn]   i
 Kutzki, Fabian [VerfasserIn]   i
 König, Gesa [VerfasserIn]   i
 Denis, Cécile V. [VerfasserIn]   i
 Gräter, Frauke [VerfasserIn]   i
 Wilmanns, Matthias [VerfasserIn]   i
 Auton, Matthew [VerfasserIn]   i
 Schneider, Stefan W. [VerfasserIn]   i
 Schneppenheim, Reinhard [VerfasserIn]   i
 Hennig, Janosch [VerfasserIn]   i
 Brehm, Maria A. [VerfasserIn]   i
Titel:Gain-of-function variant p.Pro2555Arg of von Willebrand factor increases aggregate size through altering stem dynamics
Verf.angabe:Volker Huck, Po-Chia Chen, Emma-Ruoqi Xu, Alexander Tischer, Ulrike Klemm, Camilo Aponte-Santamaría, Christian Mess, Tobias Obser, Fabian Kutzki, Gesa König, Cécile V. Denis, Frauke Gräter, Matthias Wilmanns, Matthew Auton, Stefan W. Schneider, Reinhard Schneppenheim, Janosch Hennig, Maria A. Brehm
Jahr:2022
Umfang:14 S.
Fussnoten:Online veröffentlicht: 14. April 2021 ; Gesehen am 26.10.2023
Titel Quelle:Enthalten in: Thrombosis and haemostasis
Ort Quelle:Stuttgart : Thieme, 1976
Jahr Quelle:2022
Band/Heft Quelle:122(2022), 2, Seite 226-239
ISSN Quelle:2567-689X
Abstract:The multimeric plasma glycoprotein (GP) von Willebrand factor (VWF) is best known for recruiting platelets to sites of injury during primary hemostasis. Generally, mutations in the VWF gene lead to loss of hemostatic activity and thus the bleeding disorder von Willebrand disease. By employing cone and platelet aggregometry and microfluidic assays, we uncovered a platelet GPIIb/IIIa-dependent prothrombotic gain of function (GOF) for variant p.Pro2555Arg, located in the C4 domain, leading to an increase in platelet aggregate size. We performed complementary biophysical and structural investigations using circular dichroism spectra, small-angle X-ray scattering, nuclear magnetic resonance spectroscopy, molecular dynamics simulations on the single C4 domain, and dimeric wild-type and p.Pro2555Arg constructs. C4-p.Pro2555Arg retained the overall structural conformation with minor populations of alternative conformations exhibiting increased hinge flexibility and slow conformational exchange. The dimeric protein becomes disordered and more flexible. Our data suggest that the GOF does not affect the binding affinity of the C4 domain for GPIIb/IIIa. Instead, the increased VWF dimer flexibility enhances temporal accessibility of platelet-binding sites. Using an interdisciplinary approach, we revealed that p.Pro2555Arg is the first VWF variant, which increases platelet aggregate size and shows a shear-dependent function of the VWF stem region, which can become hyperactive through mutations. Prothrombotic GOF variants of VWF are a novel concept of a VWF-associated pathomechanism of thromboembolic events, which is of general interest to vascular health but not yet considered in diagnostics. Thus, awareness should be raised for the risk they pose. Furthermore, our data implicate the C4 domain as a novel antithrombotic drug target.
DOI:doi:10.1055/a-1344-4405
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext: https://doi.org/10.1055/a-1344-4405
 Volltext: http://www.thieme-connect.de/DOI/DOI?10.1055/a-1344-4405
 DOI: https://doi.org/10.1055/a-1344-4405
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:GPIIb/IIIa
 hypercoagulability
 von Willebrand factor
K10plus-PPN:186810334X
Verknüpfungen:→ Zeitschrift

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