Increased plasma level of soluble P-selectin in non-hospitalized COVID-19 convalescent donors

• Verfasst von: Müller, Rebecca [VerfasserIn]
• Verfasst von: Rink, Gabriele [VerfasserIn]
• Verfasst von: Uzun, Günalp [VerfasserIn]
• Verfasst von: Bakchoul, Tamam [VerfasserIn]
• Verfasst von: Wuchter, Patrick [VerfasserIn]
• Verfasst von: Klüter, Harald [VerfasserIn]
• Verfasst von: Bugert, Peter [VerfasserIn]
• Titel: Increased plasma level of soluble P-selectin in non-hospitalized COVID-19 convalescent donors
• Titelzusatz: full length article
• Verf.angabe: Rebecca Müller, Gabi Rink, Günalp Uzun, Tamam Bakchoul, Patrick Wuchter, Harald Klüter, Peter Bugert
• E-Jahr: 2022
• Jahr: August 2022
• Umfang: 5 S.
• Fussnoten: Online verfügbar: 5 July 2022, Artikelversion: 7 July 2022 ; Gesehen am 30.10.2023
• Titel Quelle: Enthalten in: Thrombosis research
• Ort Quelle: Amsterdam [u.a.] : Elsevier Science, 1972
• Jahr Quelle: 2022
• Band/Heft Quelle: 216(2022) vom: Aug., Seite 120-124
• ISSN Quelle: 1879-2472
• DOI: doi:10.1016/j.thromres.2022.06.014
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: CD62P
• Sach-SW: COVID-19
• Sach-SW: Plasma marker
• Sach-SW: Platelet function
• Sach-SW: SARS-CoV-2 infection
• Sach-SW: Soluble P-selectin
• K10plus-PPN: 1868776549

Abstract

Background - The coronavirus disease-2019 (COVID-19) is a systemic disease with severe implications on the vascular and coagulation system. A procoagulant platelet phenotype has been reported at least in the acute disease phase. Soluble P-selectin (sP-sel) in the plasma is a surrogate biomarker of platelet activation. Increased plasma levels of sP-sel have been reported in hospitalized COVID-19 patients associated with disease severity. Here, we evaluated in a longitudinal study the sP-sel plasma concentration in blood donors who previously suffered from moderate COVID-19. - Methods - 154 COVID-19 convalescent and 111 non-infected control donors were recruited for plasma donation and for participation in the CORE research trial. First donation (T1) was performed 43-378 days after COVID-19 diagnosis. From most of the donors the second (T2) plasma donation including blood sampling was obtained after a time period of 21-74 days and the third (T3) donation after additional 22-78 days. Baseline characteristics including COVID-19 symptoms of the donors were recorded based on a questionnaire. Platelet function was measured at T1 by flow cytometry and light transmission aggregometry in a representative subgroup of 25 COVID-19 convalescent and 28 control donors. The sP-sel plasma concentration was determined in a total of 704 samples by using a commercial ELISA. - Results - In vitro platelet function was comparable in COVID-19 convalescent and control donors at T1. Plasma samples from COVID-19 convalescent donors revealed a significantly higher sP-sel level compared to controls at T1 (1.05 ± 0.42 ng/mL vs. 0.81 ± 0.30 ng/mL; p < 0.0001) and T2 (0.96 ± 0.39 ng/mL vs. 0.83 ± 0.38 ng/mL; p = 0.0098). At T3 the sP-sel plasma level was comparable in both study groups. Most of the COVID-19 convalescent donors showed a continuous decrease of sP-sel from T1 to T3. - Conclusion - Increased sP-sel plasma concentration as a marker for platelet or endothelial activation could be demonstrated even weeks after moderate COVID-19, whereas, in vitro platelet function was comparable with non-infected controls. We conclude that COVID-19 and additional individual factors could lead to an increase of the sP-sel plasma level.