Podocytes are the major source of IL-1α and IL-1β in human glomerulonephritides

• Verfasst von: Niemir, Zofia [VerfasserIn]
• Verfasst von: Stein, Henning [VerfasserIn]
• Verfasst von: Dworacki, Grzegorz [VerfasserIn]
• Verfasst von: Mundel, Peter [VerfasserIn]
• Verfasst von: Köhl-Hackert, Nadja [VerfasserIn]
• Verfasst von: Fröhlich, Boris Erwin [VerfasserIn]
• Verfasst von: Autschbach, Frank [VerfasserIn]
• Verfasst von: Andrassy, Konrad [VerfasserIn]
• Verfasst von: Ritz, Eberhard [VerfasserIn]
• Verfasst von: Waldherr, Rüdiger [VerfasserIn]
• Verfasst von: Otto, Herwart F. [VerfasserIn]
• Titel: Podocytes are the major source of IL-1α and IL-1β in human glomerulonephritides
• Verf.angabe: Zofia I. Niemir, Henning Stein, Grzegorz Dworacki, Peter Mundel, Nadja Koehl, Boris Koch, Frank Autschbach, Konrad Andrassy, Eberhard Ritz, Ruediger Waldherr, Herwart F. Otto
• Jahr: 1997
• Umfang: 11 S.
• Fussnoten: Artikelversion: 16. Dezember 2015 ; Gesehen am 02.11.2023
• Titel Quelle: Enthalten in: Kidney international
• Ort Quelle: New York, NY : Elsevier, 1972
• Jahr Quelle: 1997
• Band/Heft Quelle: 52(1997), 2, Seite 393-403
• ISSN Quelle: 1523-1755
• DOI: doi:10.1038/ki.1997.346
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: glomerulonephritis
• Sach-SW: interleukin
• Sach-SW: lesion
• Sach-SW: podocytes
• Sach-SW: sclerosis
• K10plus-PPN: 1868979598

Abstract

Podocytes are the major source of IL-1α and IL-1β in human glomerulonephritides. To address the question of in situ production of IL-1α and IL-1β in proliferative and non-proliferative forms of human glomerulo-nephritis (GN), we performed immunocytochemical and in situ hybridization studies on renal biopsies from patients with mesangial IgA-GN (N = 38), idiopathic membranous GN (MGN; N = 12), minimal change disease (MCD; N = 9), focal segmental glomerulosclerosis (FSGS; N = 5) and acute endocapillary GN (AGN; N = 3). Normal kidneys (N = 10) served as controls. Concomitantly, the expression of IL-1 receptor type I (IL-1 RI), IL-1 receptor type II (IL-1 RII) and of IL-1 receptor antagonist (IL-1 RA) was analyzed. Antibodies against antigens expressed on podocytes (PP-44), endothelial cells (CD31) and monocytes/macrophages (CD11b, CD14, CD68) were applied to attribute the expression of IL-1/IL-1 related peptides to intrinsic glomerular and/or blood-derived infiltrating cells. Our results demonstrate that IL-1 RII is constitutively expressed on endothelial cells, and its expression can be induced in proximal tubular cells and in the interstitium. In diseased glomeruli podocytes are capable of producing IL-1α/β. In MGN and MCD/FSGS, the expression of both IL-1 forms is particularly noted in early stages of the disease and is not only accompanied by a marked reactivity for IL-1 RI, but also for IL-1 RA. In segmental sclerosing lesions in FSGS and in IgA-GN with marked glomerular proliferation and/or sclerosis, a reduced expression of the PP-44 antigen and a diminished ability of podocytes to produce IL-1/IL-1 related peptides are noted. These results suggest that intrinsic glomerular production of IL-1 may be of relevance for the protection of glomeruli from continuing injury.