| |  Online-Ressource |
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| Verfasst von: | Pérez Bravo, David [VerfasserIn]  |
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| | Myti, Despoina [VerfasserIn] |
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| | Mižíková, Ivana [VerfasserIn] |
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| | Pfeffer, Tilman [VerfasserIn] |
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| | Surate Solaligue, David E. [VerfasserIn] |
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| | Nardiello, Claudio [VerfasserIn] |
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| | Vadász, István [VerfasserIn] |
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| | Herold, Susanne [VerfasserIn] |
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| | Seeger, Werner [VerfasserIn] |
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| | Ahlbrecht, Katrin [VerfasserIn] |
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| | Morty, Rory E. [VerfasserIn] |
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| Titel: | A comparison of airway pressures for inflation fixation of developing mouse lungs for stereological analyses |
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| Verf.angabe: | David Pérez-Bravo, Despoina Myti, Ivana Mižíková, Tilman Pfeffer, David E. Surate Solaligue, Claudio Nardiello, István Vadász, Susanne Herold, Werner Seeger, Katrin Ahlbrecht, Rory E. Morty |
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| Jahr: | 2021 |
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| Umfang: | 12 S. |
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| Illustrationen: | Illustrationen |
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| Fussnoten: | Online published: 29 December 2020 ; Gesehen am 09.11.2023 |
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| Titel Quelle: | Enthalten in: Histochemistry and cell biology |
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| Ort Quelle: | Berlin : Springer, 1958 |
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| Jahr Quelle: | 2021 |
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| Band/Heft Quelle: | 155(2021), 2, Seite 203-214 |
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| ISSN Quelle: | 1432-119X |
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| Abstract: | The morphometric analysis of lung structure using the principles of stereology has emerged as a powerful tool to describe the structural changes in lung architecture that accompany the development of lung disease that is experimentally modelled in adult mice. These stereological principles are now being applied to the study of the evolution of the lung architecture over the course of prenatal and postnatal lung development in mouse neonates and adolescents. The immature lung is structurally and functionally distinct from the adult lung, and has a smaller volume than does the adult lung. These differences have raised concerns about whether the inflation fixation of neonatal mouse lungs with the airway pressure (Paw) used for the inflation fixation of adult mouse lungs may cause distortion of the neonatal mouse lung structure, leading to the generation of artefacts in subsequent analyses. The objective of this study was to examine the impact of a Paw of 10, 20 and 30 cmH2O on the estimation of lung volumes and stereologically assessed parameters that describe the lung structure in developing mouse lungs. The data presented demonstrate that low Paw (10 cmH2O) leads to heterogeneity in the unfolding of alveolar structures within the lungs, and that high Paw (30 cmH2O) leads to an overestimation of the lung volume, and thus, affects the estimation of volume-dependent parameters, such as total alveoli number and gas-exchange surface area. Thus, these data support the use of a Paw of 20 cmH2O for inflation fixation in morphometric studies on neonatal mouse lungs. |
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| DOI: | doi:10.1007/s00418-020-01951-0 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
kostenfrei: Volltext: https://doi.org/10.1007/s00418-020-01951-0 |
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| | kostenfrei: Volltext: https://link.springer.com/article/10.1007/s00418-020-01951-0 |
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| | DOI: https://doi.org/10.1007/s00418-020-01951-0 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | Airway pressure |
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| | Inflation fixation |
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| | Lung development |
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| | Lung volume |
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| | Mouse |
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| | Stereology |
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| K10plus-PPN: | 1869909763 |
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| Verknüpfungen: | → Zeitschrift |
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¬A¬ comparison of airway pressures for inflation fixation of developing mouse lungs for stereological analyses / Pérez Bravo, David [VerfasserIn]; 2021 (Online-Ressource)
