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| Verfasst von: | Thomas, Markus [VerfasserIn]  |
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| | Felcht, Moritz [VerfasserIn] |
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| | Kruse, Karoline [VerfasserIn] |
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| | Kretschmer, Stella [VerfasserIn] |
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| | Deppermann, Carleen [VerfasserIn] |
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| | Biesdorf, Andreas [VerfasserIn] |
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| | Rohr, Karl [VerfasserIn] |
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| | Benest, Andrew V. [VerfasserIn] |
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| | Fiedler, Ulrike [VerfasserIn] |
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| | Augustin, Hellmut [VerfasserIn] |
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| Titel: | Angiopoietin-2 stimulation of endothelial cells induces αvβ3 integrin internalization and degradation |
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| Werktitel: | Angiopoietin-2 stimulation of endothelial cells induces alpha v beta 3 integrin internalization and degradation |
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| Verf.angabe: | Markus Thomas, Moritz Felcht, Karoline Kruse, Stella Kretschmer, Carleen Deppermann, Andreas Biesdorf, Karl Rohr, Andrew V. Benest, Ulrike Fiedler, and Hellmut G. Augustin |
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| E-Jahr: | 2010 |
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| Jahr: | 2 June 2010 |
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| Umfang: | 8 S. |
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| Fussnoten: | Gesehen am 15.11.2023 |
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| Titel Quelle: | Enthalten in: The journal of biological chemistry |
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| Ort Quelle: | Bethesda, Md. : ASBMB Publications, 1905 |
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| Jahr Quelle: | 2010 |
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| Band/Heft Quelle: | 285(2010), 31, Seite 23842-23849 |
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| ISSN Quelle: | 1083-351X |
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| Abstract: | The angiopoietins (Ang-1 and Ang-2) have been identified as agonistic and antagonistic ligands of the endothelial receptor tyrosine kinase Tie2, respectively. Both ligands have been demonstrated to induce translocation of Tie2 to cell-cell junctions. However, only Ang-1 induces Tie2-dependent Akt activation and subsequent survival signaling and endothelial quiescence. Ang-2 interferes negatively with Ang-1/Tie2 signaling, thereby antagonizing the Ang-1/Tie2 axis. Here, we show that both Ang-1 and Ang-2 recruit β3 integrins to Tie2. This co-localization is most prominent in cell-cell junctions. However, only Ang-2 stimulation resulted in complex formation among Tie2, αvβ3 integrin, and focal adhesion kinase as evidenced by co-immunoprecipitation experiments. Focal adhesion kinase was phosphorylated in the FAT domain at Ser910 upon Ang-2 stimulation and the adaptor proteins p130Cas and talin dissociated from αvβ3 integrin. The αvβ3 integrin was internalized, ubiquitinylated, and gated toward lysosomes. Taken together, the experiments define Tie2/αvβ3 integrin association-induced integrin internalization and degradation as mechanistic consequences of endothelial Ang-2 stimulation. |
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| DOI: | doi:10.1074/jbc.M109.097543 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1074/jbc.M109.097543 |
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| | Volltext: https://www.sciencedirect.com/science/article/pii/S0021925820618506 |
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| | DOI: https://doi.org/10.1074/jbc.M109.097543 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | Angiopoietin |
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| | Cell Adhesion |
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| | Cell Junctions |
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| | Endothelium |
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| | Integrin |
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| | Protein-tyrosine Kinase (Tyrosine Kinase) |
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| | Tie |
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| | Vascular Biology |
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| K10plus-PPN: | 1870346769 |
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| Verknüpfungen: | → Zeitschrift |
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Angiopoietin-2 stimulation of endothelial cells induces αvβ3 integrin internalization and degradation / Thomas, Markus [VerfasserIn]; 2 June 2010 (Online-Ressource)
