Approaches to characterize chromatin subcompartment organization in the cell nucleus

• Verfasst von: Weinmann, Robin [VerfasserIn]
• Verfasst von: Frank, Lukas [VerfasserIn]
• Verfasst von: Rippe, Karsten [VerfasserIn]
• Titel: Approaches to characterize chromatin subcompartment organization in the cell nucleus
• Verf.angabe: Robin Weinmann, Lukas Frank and Karsten Rippe
• E-Jahr: 2023
• Jahr: December 2023
• Umfang: 7 S.
• Fussnoten: Online veröffentlicht: 16. September 2023 ; Gesehen am 21.11.2023
• Weitere Titel: Titel des übergeordneten Themenheftes: 3D genome chromatin organization and regulation
• Titel Quelle: Enthalten in: Current opinion in structural biology
• Ort Quelle: Amsterdam [u.a.] : Elsevier, 1991
• Jahr Quelle: 2023
• Band/Heft Quelle: 83(2023) vom: Okt., Artikel-ID 102695, Seite 1-7
• DOI: doi:10.1016/j.sbi.2023.102695
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: Chromatin subcompartment
• Sach-SW: Genome activities
• Sach-SW: Nuclear organization
• Sach-SW: Phase separation
• Sach-SW: Transcription factor
• K10plus-PPN: 1870770129

Abstract

The mechanism of self-organization of chromatin subcompartments on the 0.1-1 μm scale and their impact on genome-associated activities has long been a key aspect of research on nuclear organization. Understanding the underlying structure-function relationship, however, remains challenging due to the complex hierarchical structure of chromatin and the polymorphic organization of subcompartments that assemble around it. Towards this goal, approaches to measure local properties and compositional dynamics of chromatin in its endogenous cellular environment are instrumental. Here, we discuss recent advancements in studying these features and their functional implications in protein and RNA enrichment and genome accessibility.