Pervasive prophage recombination occurs during evolution of spore-forming Bacilli

• Verfasst von: Dragoš, Anna [VerfasserIn]
• Verfasst von: Priyadarshini, B. [VerfasserIn]
• Verfasst von: Hasan, Zahraa [VerfasserIn]
• Verfasst von: Strube, Mikael Lenz [VerfasserIn]
• Verfasst von: Kempen, Paul J. [VerfasserIn]
• Verfasst von: Maróti, Gergely [VerfasserIn]
• Verfasst von: Kaspar, Charlotte [VerfasserIn]
• Verfasst von: Bose, Baundauna [VerfasserIn]
• Verfasst von: Burton, Briana M. [VerfasserIn]
• Verfasst von: Bischofs-Pfeifer, Ilka [VerfasserIn]
• Verfasst von: Kovács, Ákos T. [VerfasserIn]
• Titel: Pervasive prophage recombination occurs during evolution of spore-forming Bacilli
• Verf.angabe: Anna Dragoš, B. Priyadarshini, Zahraa Hasan, Mikael Lenz Strube, Paul J. Kempen, Gergely Maróti, Charlotte Kaspar, Baundauna Bose, Briana M. Burton, Ilka B. Bischofs, Ákos T. Kovács
• Jahr: 2021
• Umfang: 15 S.
• Illustrationen: Illustrationen
• Fussnoten: Published online: 20 December 2020 ; Gesehen am 23.11.2023
• Titel Quelle: Enthalten in: International Society for Microbial EcologyThe ISME journal
• Ort Quelle: Basingstoke : Nature Publishing Group, 2007
• Jahr Quelle: 2021
• Band/Heft Quelle: 15(2021), 5, Seite 1344-1358
• ISSN Quelle: 1751-7370
• DOI: doi:10.1038/s41396-020-00854-1
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: Bacterial genetics
• Sach-SW: Evolution
• K10plus-PPN: 1871030005

Abstract

Phages are the main source of within-species bacterial diversity and drivers of horizontal gene transfer, but we know little about the mechanisms that drive genetic diversity of these mobile genetic elements (MGEs). Recently, we showed that a sporulation selection regime promotes evolutionary changes within SPβ prophage of Bacillus subtilis, leading to direct antagonistic interactions within the population. Herein, we reveal that under a sporulation selection regime, SPβ recombines with low copy number phi3Ts phage DNA present within the B. subtilis population. Recombination results in a new prophage occupying a different integration site, as well as the spontaneous release of virulent phage hybrids. Analysis of Bacillus sp. strains suggests that SPβ and phi3T belong to a distinct cluster of unusually large phages inserted into sporulation-related genes that are equipped with a spore-related genetic arsenal. Comparison of Bacillus sp. genomes indicates that similar diversification of SPβ-like phages takes place in nature. Our work is a stepping stone toward empirical studies on phage evolution, and understanding the eco-evolutionary relationships between bacteria and their phages. By capturing the first steps of new phage evolution, we reveal striking relationship between survival strategy of bacteria and evolution of their phages.