| |  Online-Ressource |
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| Verfasst von: | Gleitze, Silvia [VerfasserIn]  |
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| | Ramirez, Omar [VerfasserIn] |
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| | Vega-Vásquez, Ignacio [VerfasserIn] |
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| | Yan, Jing [VerfasserIn] |
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| | Lobos, Pedro [VerfasserIn] |
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| | Bading, Hilmar [VerfasserIn] |
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| | Núñez, Marco T. [VerfasserIn] |
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| | Paula-Lima, Andrea [VerfasserIn] |
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| | Hidalgo, Cecilia [VerfasserIn] |
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| Titel: | Ryanodine receptor mediated calcium release contributes to ferroptosis induced in primary hippocampal neurons by GPX4 inhibition |
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| Verf.angabe: | Silvia Gleitze, Omar A. Ramírez, Ignacio Vega-Vásquez, Jing Yan, Pedro Lobos, Hilmar Bading, Marco T. Núñez, Andrea Paula-Lima and Cecilia Hidalgo |
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| E-Jahr: | 2023 |
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| Jahr: | 13 March 2023 |
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| Umfang: | 18 S. |
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| Fussnoten: | Gesehen am 24.11.2023 |
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| Titel Quelle: | Enthalten in: Antioxidants |
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| Ort Quelle: | Basel : MDPI, 2013 |
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| Jahr Quelle: | 2023 |
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| Band/Heft Quelle: | 12(2023), 3, Artikel-ID 705, Seite 1-18 |
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| ISSN Quelle: | 2076-3921 |
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| Abstract: | Ferroptosis, a newly described form of regulated cell death, is characterized by the iron-dependent accumulation of lipid peroxides, glutathione depletion, mitochondrial alterations, and enhanced lipoxygenase activity. Inhibition of glutathione peroxidase 4 (GPX4), a key intracellular antioxidant regulator, promotes ferroptosis in different cell types. Scant information is available on GPX4-induced ferroptosis in hippocampal neurons. Moreover, the role of calcium (Ca2+) signaling in ferroptosis remains elusive. Here, we report that RSL3, a selective inhibitor of GPX4, caused dendritic damage, lipid peroxidation, and induced cell death in rat primary hippocampal neurons. Previous incubation with the ferroptosis inhibitors deferoxamine or ferrostatin-1 reduced these effects. Likewise, preincubation with micromolar concentrations of ryanodine, which prevent Ca2+ release mediated by Ryanodine Receptor (RyR) channels, partially protected against RSL3-induced cell death. Incubation with RSL3 for 24 h suppressed the cytoplasmic Ca2+ concentration increase induced by the RyR agonist caffeine or by the SERCA inhibitor thapsigargin and reduced hippocampal RyR2 protein content. The present results add to the current understanding of ferroptosis-induced neuronal cell death in the hippocampus and provide new information both on the role of RyR-mediated Ca2+ signals on this process and on the effects of GPX4 inhibition on endoplasmic reticulum calcium content. |
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| DOI: | doi:10.3390/antiox12030705 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.3390/antiox12030705 |
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| | Volltext: https://www.mdpi.com/2076-3921/12/3/705 |
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| | DOI: https://doi.org/10.3390/antiox12030705 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | calcium signaling |
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| | cell death |
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| | dendritic morphology |
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| | endoplasmic reticulum |
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| | ferroptosis |
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| | glutathione peroxidase |
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| | iron |
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| | iron chelators |
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| | lipid peroxidation |
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| | neurodegeneration |
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| | oxidative stress |
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| | reactive oxygen species |
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| | RSL3 |
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| K10plus-PPN: | 187104443X |
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| Verknüpfungen: | → Zeitschrift |
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Ryanodine receptor mediated calcium release contributes to ferroptosis induced in primary hippocampal neurons by GPX4 inhibition / Gleitze, Silvia [VerfasserIn]; 13 March 2023 (Online-Ressource)
