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| Verfasst von: | Valeriano Nunes, Ivanéia [VerfasserIn]  |
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| | Andrade, Camila Mattos [VerfasserIn] |
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| | Guerra, Priscila Valera [VerfasserIn] |
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| | Khouri, Mariana Ivo [VerfasserIn] |
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| | Galantini, Maria Poliana Leite [VerfasserIn] |
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| | da Silva, Robson Amaro Augusto [VerfasserIn] |
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| | Faquim-Mauro, Eliana L. [VerfasserIn] |
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| | Farias, Leonardo Paiva [VerfasserIn] |
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| | Rebouças, Juliana de Souza [VerfasserIn] |
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| | Faria, Ana Maria Caetano [VerfasserIn] |
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| | Brodskyn, Cláudia Ida [VerfasserIn] |
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| Titel: | A new experimental model to study shrimp allergy |
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| Verf.angabe: | Ivanéia Valeriano Nunes, Camila Mattos Andrade, Priscila Valera Guerra, Mariana Ivo Khouri, Maria Poliana Leite Galantini, Robson Amaro Augusto da Silva, Eliana L. Faquim-Mauro, Leonardo Paiva Farias, Juliana de Souza Rebouças, Ana Maria Caetano Faria, Cláudia Ida Brodskyn |
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| E-Jahr: | 2023 |
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| Jahr: | August 2023 |
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| Umfang: | 8 S. |
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| Illustrationen: | Illustrationen |
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| Fussnoten: | Gesehen am 28.11.2023 ; Online verfügbar: 13. Juni 2023, Version des Artikels: 5. Juli 2023 |
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| Titel Quelle: | Enthalten in: Immunology letters |
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| Ort Quelle: | Amsterdam [u.a.] : Elsevier Science, 1979 |
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| Jahr Quelle: | 2023 |
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| Band/Heft Quelle: | 260(2023), Seite 73-80 |
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| ISSN Quelle: | 1879-0542 |
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| Abstract: | Shrimp is among the most sensitizing food allergens and has been associated with many anaphylaxis reactions. However, there is still a shortage of studies that enable a systematic understanding of this disease and the investigation of new therapeutic approaches. This study aimed to develop a new experimental model of shrimp allergy that could enable the evaluation of new prophylactic treatments. BALB/c mice were subcutaneously sensitized with 100 μg of shrimp proteins of Litopenaeus vannamei adsorbed in 1 mg of aluminum hydroxide on day 0, and a booster (100 µg of shrimp proteins only) on day 14. The oral challenge protocol was based on the addition of 5 mg/ml of shrimp proteins to water from day 21 to day 35. Analysis of shrimp extract content detected at least 4 of the major allergens reported to L. vannamei. In response to the sensitization, allergic mice showed significantly enhanced IL-4 and IL-10 production in restimulated cervical draining lymph node cells. High detection of serum anti-shrimp IgE and IgG1 suggested the development of allergies to shrimp while Passive Cutaneous Anaphylaxis assay revealed an IgE-mediated response. Immunoblotting analysis revealed that Allergic mice developed antibodies to multiple antigens present in the shrimp extract. These observations were supported by the detection of anti-shrimp IgA production in intestinal lavage samples and morphometric intestinal mucosal changes. Therefore, this experimental protocol can be a tool to evaluate prophylactic and therapeutic approaches. |
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| DOI: | doi:10.1016/j.imlet.2023.06.007 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
kostenfrei: Volltext: https://doi.org/10.1016/j.imlet.2023.06.007 |
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| | kostenfrei: Volltext: https://www.sciencedirect.com/science/article/pii/S0165247823001098 |
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| | DOI: https://doi.org/10.1016/j.imlet.2023.06.007 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | Eosinophils |
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| | Food allergy |
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| | IgE |
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| | Intestinal mucosa |
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| | Shrimp allergy |
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| K10plus-PPN: | 1871510597 |
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| Verknüpfungen: | → Zeitschrift |
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¬A¬ new experimental model to study shrimp allergy / Valeriano Nunes, Ivanéia [VerfasserIn]; August 2023 (Online-Ressource)
