| Online-Ressource |
Verfasst von: | Valeriano Nunes, Ivanéia [VerfasserIn]  |
| Andrade, Camila Mattos [VerfasserIn]  |
| Guerra, Priscila Valera [VerfasserIn]  |
| Khouri, Mariana Ivo [VerfasserIn]  |
| Galantini, Maria Poliana Leite [VerfasserIn]  |
| da Silva, Robson Amaro Augusto [VerfasserIn]  |
| Faquim-Mauro, Eliana L. [VerfasserIn]  |
| Farias, Leonardo Paiva [VerfasserIn]  |
| Rebouças, Juliana de Souza [VerfasserIn]  |
| Faria, Ana Maria Caetano [VerfasserIn]  |
| Brodskyn, Cláudia Ida [VerfasserIn]  |
Titel: | A new experimental model to study shrimp allergy |
Verf.angabe: | Ivanéia Valeriano Nunes, Camila Mattos Andrade, Priscila Valera Guerra, Mariana Ivo Khouri, Maria Poliana Leite Galantini, Robson Amaro Augusto da Silva, Eliana L. Faquim-Mauro, Leonardo Paiva Farias, Juliana de Souza Rebouças, Ana Maria Caetano Faria, Cláudia Ida Brodskyn |
E-Jahr: | 2023 |
Jahr: | August 2023 |
Umfang: | 8 S. |
Illustrationen: | Illustrationen |
Fussnoten: | Gesehen am 28.11.2023 ; Online verfügbar: 13. Juni 2023, Version des Artikels: 5. Juli 2023 |
Titel Quelle: | Enthalten in: Immunology letters |
Ort Quelle: | Amsterdam [u.a.] : Elsevier Science, 1979 |
Jahr Quelle: | 2023 |
Band/Heft Quelle: | 260(2023), Seite 73-80 |
ISSN Quelle: | 1879-0542 |
Abstract: | Shrimp is among the most sensitizing food allergens and has been associated with many anaphylaxis reactions. However, there is still a shortage of studies that enable a systematic understanding of this disease and the investigation of new therapeutic approaches. This study aimed to develop a new experimental model of shrimp allergy that could enable the evaluation of new prophylactic treatments. BALB/c mice were subcutaneously sensitized with 100 μg of shrimp proteins of Litopenaeus vannamei adsorbed in 1 mg of aluminum hydroxide on day 0, and a booster (100 µg of shrimp proteins only) on day 14. The oral challenge protocol was based on the addition of 5 mg/ml of shrimp proteins to water from day 21 to day 35. Analysis of shrimp extract content detected at least 4 of the major allergens reported to L. vannamei. In response to the sensitization, allergic mice showed significantly enhanced IL-4 and IL-10 production in restimulated cervical draining lymph node cells. High detection of serum anti-shrimp IgE and IgG1 suggested the development of allergies to shrimp while Passive Cutaneous Anaphylaxis assay revealed an IgE-mediated response. Immunoblotting analysis revealed that Allergic mice developed antibodies to multiple antigens present in the shrimp extract. These observations were supported by the detection of anti-shrimp IgA production in intestinal lavage samples and morphometric intestinal mucosal changes. Therefore, this experimental protocol can be a tool to evaluate prophylactic and therapeutic approaches. |
DOI: | doi:10.1016/j.imlet.2023.06.007 |
URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
kostenfrei: Volltext: https://doi.org/10.1016/j.imlet.2023.06.007 |
| kostenfrei: Volltext: https://www.sciencedirect.com/science/article/pii/S0165247823001098 |
| DOI: https://doi.org/10.1016/j.imlet.2023.06.007 |
Datenträger: | Online-Ressource |
Sprache: | eng |
Sach-SW: | Eosinophils |
| Food allergy |
| IgE |
| Intestinal mucosa |
| Shrimp allergy |
K10plus-PPN: | 1871510597 |
Verknüpfungen: | → Zeitschrift |
¬A¬ new experimental model to study shrimp allergy / Valeriano Nunes, Ivanéia [VerfasserIn]; August 2023 (Online-Ressource)