Neurochemistry, connectivity and plasticity of small intensely fluorescent (SIF) cells in the rat superior cervical ganglion

• Verfasst von: Heym, Christine [VerfasserIn]
• Verfasst von: Common, Bernd [VerfasserIn]
• Verfasst von: Yin, Shui [VerfasserIn]
• Verfasst von: Klimaschewski, Lars Peter [VerfasserIn]
• Verfasst von: Couraud, Jean-Yves [VerfasserIn]
• Verfasst von: Bachmann, Sebastian [VerfasserIn]
• Titel: Neurochemistry, connectivity and plasticity of small intensely fluorescent (SIF) cells in the rat superior cervical ganglion
• Verf.angabe: Christine Heym, Bernd Common, Shui Yin, Lars Klimaschewski, Jean-Yves Couraud, Sebastian Bachmann
• Jahr: 1993
• Umfang: 11 S.
• Fussnoten: Elektronische Reproduktion der Druck-Ausgabe 1. November 2011 ; Gesehen am 30.11.2023
• Titel Quelle: Enthalten in: Annals of anatomy
• Ort Quelle: Jena : Elsevier, 1992
• Jahr Quelle: 1993
• Band/Heft Quelle: 175(1993), 4, Seite 309-319
• ISSN Quelle: 1618-0402
• DOI: doi:10.1016/S0940-9602(11)80026-9
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: Biogenic monoamines
• Sach-SW: Immunocytochemistry
• Sach-SW: In situ hybridisation
• Sach-SW: Neuropeptides
• Sach-SW: Rat
• Sach-SW: SIF cells
• Sach-SW: Superior cervical ganglion
• K10plus-PPN: 1871707986

Abstract

Applying double-labelling immunofluorescence, the peptide content of solitary and clustered small intensely fluorescent (SIF) cells, identified by an antiserum to a selective membrane glycoprotein marker, synaptophysin, was correlated with the presence of catecholamines in the rat superior cervical ganglion. Most of synaptophysin-immunoreactive solitary and clustered SIF cells apparently contained dopami-ne (indicated by tyrosine hydroxylase — TH) but not norad-renaline (indicated by dopamine-β-hydroxylase-DBH). Frequently, immunoreactivities for substance P or rarely, neuropeptide Y were colocalized in TH-immunolabelled cells of both types. Immunostaining for vasoactive intestinal polypeptide was found only in solitary SIF cells and was visible in TH-immunoreactive, as well as in TH-nonreactive cells. Very few solitary SIF cells were TH- and DBH-immu-noreactive. Solitary and clustered SIF cells, as a rule, were encircled by leu-enkephalin-positive fibres which were also met-enkephalin-arg6-phe7-immunoreactive, indicating proenkephalin as precursor. SIF cells were additionally approached by varicose fibres which contained immunoreactivity for calcitonin gene-related peptide (CGRP) but not for enkephalins. As observed by immuno-electronmicroscopy, fibres that were immunostained for leu-enkephalin or CGRP, deeply invaginated into SIF cell somata. In addition to close membrane appositions, CGRP-immunolabelled fibres exhibited efferent synaptic contacts wih elements of SIF cell clusters. SIF cells were non-reactive to enkephalin-antisera in control ganglia and after transection of the postganglionic nerves (axotomy); but both types exhibited leu-enkephalin in pre-ganglionically transected ganglia (decentralization) in which enkephalin-immunoreactive fibre baskets were absent. Synthesis of enkephalin in SIF cells after decentralization was confirmed by in situ hybridization demonstrating intracyto-plasmic proenkephalin messenger-RNA. The findings are indicative for a differential neurochemical equipment of SIF cells in the rat superior cervical ganglion, which mainly is independent to a topographical classification. Moreover, they demonstrate the involvement of two neuropeptides in preganglionic SIF cell innervation. Finally, the observations indicate the capacity of SIF cells for proen-kephalin-expression in response to preganglionic denervati-on.