Myosin V facilitates polarised E-cadherin secretion

• Verfasst von: Tanasić, Dajana [VerfasserIn]
• Verfasst von: Berns, Nicola [VerfasserIn]
• Verfasst von: Riechmann, Veit [VerfasserIn]
• Titel: Myosin V facilitates polarised E-cadherin secretion
• Verf.angabe: Dajana Tanasic, Nicola Berns, Veit Riechmann
• E-Jahr: 2022
• Jahr: July 2022
• Umfang: 17 S.
• Illustrationen: Illustrationen
• Fussnoten: First published: 16 May 2022 ; Gesehen am 19.12.2023
• Titel Quelle: Enthalten in: Traffic
• Ort Quelle: Oxford : Wiley-Blackwell, 2000
• Jahr Quelle: 2022
• Band/Heft Quelle: 23(2022), 7 vom: Juli, Seite 374-390
• ISSN Quelle: 1600-0854
• DOI: doi:10.1111/tra.12846
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: E-cadherin
• Sach-SW: Myosin V
• Sach-SW: Rab11
• Sach-SW: Rab7
• Sach-SW: SNX16
• K10plus-PPN: 1876370254

Abstract

E-cadherin has a fundamental role in epithelial tissues by providing cell-cell adhesion. Polarised E-cadherin exocytosis to the lateral plasma membrane is central for cell polarity and epithelial homeostasis. Loss of E-cadherin secretion compromises tissue integrity and is a prerequisite for metastasis. Despite this pivotal role of E-cadherin secretion, the transport mechanism is still unknown. Here we identify Myosin V as the motor for E-cadherin secretion. Our data reveal that Myosin V and F-actin are required for the formation of a continuous apicolateral E-cadherin belt, the zonula adherens. We show by live imaging how Myosin V transports E-cadherin vesicles to the plasma membrane, and distinguish two distinct transport tracks: an apical actin network leading to the zonula adherens and parallel actin bundles leading to the basal-most region of the lateral membrane. E-cadherin secretion starts in endosomes, where Rab11 and Sec15 recruit Myosin V for transport to the zonula adherens. We also shed light on the endosomal sorting of E-cadherin by showing how Rab7 and Snx16 cooperate in moving E-cadherin into the Rab11 compartment. Thus, our data help to understand how polarised E-cadherin secretion maintains epithelial architecture and prevents metastasis.