IgE binding capacity of apple allergens preserved after high pressure treatment

• Verfasst von: Fernández, Avelina [VerfasserIn]
• Verfasst von: Butz, Peter [VerfasserIn]
• Verfasst von: Tauscher, Bernhard [VerfasserIn]
• Titel: IgE binding capacity of apple allergens preserved after high pressure treatment
• Verf.angabe: Avelina Fernández, Peter Butz, Bernhard Tauscher
• E-Jahr: 2009
• Jahr: 15 Dec 2009
• Umfang: 8 S.
• Fussnoten: Gesehen am 03.01.2023
• Weitere Titel: Titel des übergeordneten Kongressbandes: Proceedings of the XLVIIth European High Pressure Research Group (EHPRG 47) meeting, Paris (France), 6-11 September 2009
• Titel Quelle: Enthalten in: High pressure research
• Ort Quelle: London [u.a.] : Taylor and Francis, 1988
• Jahr Quelle: 2009
• Band/Heft Quelle: 29(2009), 4, Seite 705-712
• ISSN Quelle: 1477-2299
• DOI: doi:10.1080/08957950903384990
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: apple allergens
• Sach-SW: high pressure
• Sach-SW: immunoblots
• Sach-SW: Mal d1
• Sach-SW: RAST
• Sach-SW: specific sera
• K10plus-PPN: 1877157074

Abstract

Two strategies were performed to assay the antigenicity of an apple extract (Golden Delicious) after combined pressure/temperature treatments using pools of sera of patients with positive serum IgE to apple. The IgE binding capacity of SDS denatured proteins was tested after electro-transference to membranes. Additionally, competitive RAST inhibition tests were carried out with the native apple proteins. Antigenicity was also assessed after 10 months of storage. In general, the results with sera of Spanish patients obtained by the RAST inhibition method confirmed the negligible effects of high pressure treatments up to 800 MPa on apple antigenicity and IgE binding, similarly to other European populations. Immunoblots revealed, contrarily, that heat is more important than pressure in inducing irreversible changes in key epitopes. Furthermore, pressure treatments (up to 800 MPa) preserved inhibition scores in frozen apple extracts closer to the untreated controls during at least 10 months, which could be relevant in the design of strategies to preserve allergen antigenicity.