Imatinib mesylate (Glivec) inhibits Schwann cell viability and reduces the size of human plexiform neurofibroma in a xenograft model

• Verfasst von: Demestre, Maria [VerfasserIn]
• Verfasst von: Herzberg, Jan [VerfasserIn]
• Verfasst von: Holtkamp, Nikola [VerfasserIn]
• Verfasst von: Hagel, Christian [VerfasserIn]
• Verfasst von: Reuss, David [VerfasserIn]
• Verfasst von: Friedrich, Reinhard E. [VerfasserIn]
• Verfasst von: Kluwe, Lan [VerfasserIn]
• Verfasst von: Deimling, Andreas von [VerfasserIn]
• Verfasst von: Mautner, Victor Felix [VerfasserIn]
• Verfasst von: Kurtz, Andreas [VerfasserIn]
• Titel: Imatinib mesylate (Glivec) inhibits Schwann cell viability and reduces the size of human plexiform neurofibroma in a xenograft model
• Verf.angabe: Maria Demestre, Jan Herzberg, Nikola Holtkamp, Christian Hagel, David Reuss, Reinhard E. Friedrich, Lan Kluwe, Andreas von Deimling, Victor-F. Mautner, Andreas Kurtz
• Jahr: 2010
• Umfang: 9 S.
• Fussnoten: Online veröffentlicht: 17 November 2009 ; Gesehen am 05.01.2024
• Titel Quelle: Enthalten in: Journal of neuro-oncology
• Ort Quelle: Dordrecht [u.a.] : Springer Science + Business Media B.V, 1983
• Jahr Quelle: 2010
• Band/Heft Quelle: 98(2010), 1, Seite 11-19
• ISSN Quelle: 1573-7373
• DOI: doi:10.1007/s11060-009-0049-4
• Datenträger: Online-Ressource
• Sprache: eng
• Bibliogr. Hinweis: Erscheint auch als : Druck-Ausgabe: Imatinib mesylate (Glivec) inhibits Schwann cell viability and reduces the size of human plexiform neurofibroma in a xenograft model. - 2010
• Sach-SW: Adolescent
• Sach-SW: Adult
• Sach-SW: Animals
• Sach-SW: Benzamides
• Sach-SW: Brain Neoplasms
• Sach-SW: Cell Line, Tumor
• Sach-SW: Cell Size
• Sach-SW: Cell Survival
• Sach-SW: Disease Models, Animal
• Sach-SW: Dose-Response Relationship, Drug
• Sach-SW: Female
• Sach-SW: Gene Expression Regulation, Neoplastic
• Sach-SW: Humans
• Sach-SW: Imatinib Mesylate
• Sach-SW: Ki-67 Antigen
• Sach-SW: Male
• Sach-SW: Mice
• Sach-SW: Mice, Inbred BALB C
• Sach-SW: Mice, Nude
• Sach-SW: Middle Aged
• Sach-SW: Neoplasm Transplantation
• Sach-SW: Neurofibroma, Plexiform
• Sach-SW: Piperazines
• Sach-SW: Protein Kinase Inhibitors
• Sach-SW: Pyrimidines
• Sach-SW: Receptor, Platelet-Derived Growth Factor alpha
• Sach-SW: Receptor, Platelet-Derived Growth Factor beta
• Sach-SW: S100 Proteins
• Sach-SW: Schwann Cells
• Sach-SW: Young Adult
• K10plus-PPN: 1877359076

Abstract

Plexiform neurofibromas (PNF), one of the major features of neurofibromatosis type 1 (NF1), are characterized by complex cellular composition and mostly slow but variable growth patterns. In this study, we examined the effect of imatinib mesylate, a receptor tyrosine kinase inhibitor, on PNF-derived Schwann cells and PNF tumour growth in vitro and in vivo. In vitro, PNF-derived primary Schwann cells express platelet-derived growth factors receptors (PDGFR) alpha and beta, both targets of imatinib, and cell viability was reduced by imatinib mesylate, with 50% inhibition concentration (IC(50)) of 10 microM. For in vivo studies, PNF tumour fragments xenografted onto the sciatic nerve of athymic nude mice were first characterized. The tumours persisted for at least 63 days and maintained typical characteristics of PNFs such as complex cellular composition, low proliferation rate and angiogenesis. A transient enlargement of the graft size was due to inflammation by host cells. Treatment with imatinib mesylate at a daily dose of 75 mg/kg for 4 weeks reduced the graft size by an average of 80% (n = 8), significantly different from the original sizes within the group and from sizes of the grafts in 11 untreated mice in the control group (P < 0.001). We demonstrated that grafting human PNF tumour fragments into nude mice provides an adequate in vivo model for drug testing. Our results provide in vivo and in vitro evidence for efficacy of imatinib mesylate for PNF.