Anti-benzo(a)pyrene diolepoxide

• Verfasst von: Rojas, Margarita [VerfasserIn]
• Verfasst von: Alexandrov, Kroum [VerfasserIn]
• Verfasst von: Auburtin, Guy [VerfasserIn]
• Verfasst von: Wastiaux-Denamur, Agnès [VerfasserIn]
• Verfasst von: Mayer, Lucienne [VerfasserIn]
• Verfasst von: Mahieu, Bernard [VerfasserIn]
• Verfasst von: Sebastien, Patrick [VerfasserIn]
• Verfasst von: Bartsch, Helmut [VerfasserIn]
• Titel: Anti-benzo(a)pyrene diolepoxide
• Titelzusatz: DNA adduct levels in peripheral mononuclear cells from coke oven workers and the enhancing effect of smoking
• Verf.angabe: Margarita Rojas, Kroum Alexandrov, Guy Auburtin, Agnès Wastiaux-Denamur, Lucienne Mayer, Bernard Mahieu, Patrick Sebastien, Helmut Bartsch
• E-Jahr: 1995
• Jahr: 01 June 1995
• Umfang: 4 S.
• Fussnoten: Im Titel steht (a) in eckiger Klammer ; Gesehen am 11.01.2024
• Titel Quelle: Enthalten in: Carcinogenesis
• Ort Quelle: Oxford : Oxford Univ. Press, 1980
• Jahr Quelle: 1995
• Band/Heft Quelle: 16(1995), 6, Seite 1373-1376
• ISSN Quelle: 1460-2180
• DOI: doi:10.1093/carcin/16.6.1373
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1877935751

Abstract

The level of (±)-r-7, t-8-dihydroxy-t-9, 10-oxy-7,8,9,10-tetrahydrobenzo[a]pyrene (anti-BPDE) bound to DNA of lymphocytes plus monocytes in 39 coke oven workers exposed to polycyclic aromatic hydrocarbons (PAH) and 39 non-exposed persons (controls) were investigated, each of the groups consisting of smokers and non-smokers. The adduct level was measured by an improved HPLC/fluorescence method (Rojas, M., Alexandrov, K., van Schooten, F.J., Hillebrand, M., Kriek, E. and Bartsch, H., Carcinogenesis, 15, 557-560, 1994) through the release of the corresponding benzo[a]pyrene (B[a]P) tetrols. The anti-BPDE-DNA adduct was detected in 51% of coke oven workers exposed to PAH and in 18% of the non-exposed (control) subjects. The mean level of anti-BPDE-DNA adducts/108 nucleotides in coke oven workers (15.7 ± 37.8) was ∼8 times higher than in non-exposed subjects (2.0 ± 8.7). The interindividual variation of adduct levels was ∼ 100-fold in coke oven workers and ∼50-fold in controls respectively. Smokers in the exposed group had 3.5 times more DNA adducts than non-smokers. With the exception of one non-smoker with very high adduct levels (52.8 adducts/108), the control subjects showed the presence of barely detectable adducts in only 16% of the samples examined. The increased in vivo formation in some smokers and high variability of anti-BPDE-DNA adducts in coke oven workers suggests variations in genetically controlled activation/inactivation reactions of PAH metabolism.