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| Verfasst von: | Beine-Golovchuk, Olga [VerfasserIn]  |
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| | Kallas, Martina [VerfasserIn] |
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| | Kunze, Ruth [VerfasserIn] |
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| | Griesel, Sabine [VerfasserIn] |
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| | Baßler, Jochen [VerfasserIn] |
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| Titel: | The Efg1-Bud22 dimer associates with the U14 snoRNP contacting the 5′ rRNA domain of an early 90S pre-ribosomal particle |
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| Verf.angabe: | Olga Beine-Golovchuk, Martina Kallas, Ruth Kunze, Sabine Griesel and Jochen Baßler |
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| E-Jahr: | 2024 |
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| Jahr: | January 2024 |
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| Umfang: | 17 S. |
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| Fussnoten: | Gesehen am 23.01.2024 ; Online vorab veröffentlicht: 24. November 2023 |
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| Titel Quelle: | Enthalten in: Nucleic acids research |
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| Ort Quelle: | Oxford : Oxford Univ. Press, 1974 |
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| Jahr Quelle: | 2024 |
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| Band/Heft Quelle: | 52(2024), 21 vom: Jan., Seite 431-447 |
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| ISSN Quelle: | 1362-4962 |
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| Abstract: | The DEAD-box helicase Dbp4 plays an essential role during the early assembly of the 40S ribosome, which is only poorly understood to date. By applying the yeast two-hybrid method and biochemical approaches, we discovered that Dbp4 interacts with the Efg1-Bud22 dimer. Both factors associate with early pre-90S particles and smaller complexes, each characterized by a high presence of the U14 snoRNA. A crosslink analysis of Bud22 revealed its contact to the U14 snoRNA and the 5 ' domain of the nascent 18S rRNA, close to its U14 snoRNA hybridization site. Moreover, depletion of Bud22 or Efg1 specifically affects U14 snoRNA association with pre-ribosomal complexes. Accordingly, we concluded that the role of the Efg1-Bud22 dimer is linked to the U14 snoRNA function on early 90S ribosome intermediates chaperoning the 5 ' domain of the nascent 18S rRNA. The successful rRNA folding of the 5 ' domain and the release of Efg1, Bud22, Dpb4, U14 snoRNA and associated snoRNP factors allows the subsequent recruitment of the Kre33-Bfr2-Enp2-Lcp5 module towards the 90S pre-ribosome. Graphical Abstract |
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| DOI: | doi:10.1093/nar/gkad1109 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
kostenfrei: Volltext: https://doi.org/10.1093/nar/gkad1109 |
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| | kostenfrei: Volltext: https://www.webofscience.com/api/gateway?GWVersion=2&SrcAuth=DynamicDOIArticle&SrcApp=WOS&KeyAID=10.1093%2Fnar%2Fgkad110 ... |
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| | DOI: https://doi.org/10.1093/nar/gkad1109 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | BIOGENESIS |
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| | HELICASE DBP4 |
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| | IDENTIFICATION |
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| | PRERIBOSOME |
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| | PROCESSOME |
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| | PROTEINS |
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| | SMALL NUCLEOLAR RNA |
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| | STEPWISE |
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| | U3 SNORNA |
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| | VERSATILE |
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| K10plus-PPN: | 1878675001 |
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| Verknüpfungen: | → Zeitschrift |
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¬The¬ Efg1-Bud22 dimer associates with the U14 snoRNP contacting the 5′ rRNA domain of an early 90S pre-ribosomal particle / Beine-Golovchuk, Olga [VerfasserIn]; January 2024 (Online-Ressource)
