Integrated proteome and malonylome analyses reveal the neutrophil extracellular trap formation pathway in rheumatoid arthritis

• Verfasst von: Hu, Biying [VerfasserIn]
• Verfasst von: Li, Dandan [VerfasserIn]
• Verfasst von: Zeng, Zhipeng [VerfasserIn]
• Verfasst von: Zhang, Zeyu [VerfasserIn]
• Verfasst von: Cao, Rui [VerfasserIn]
• Verfasst von: Dong, XiangNan [VerfasserIn]
• Verfasst von: Yun, Chen [VerfasserIn]
• Verfasst von: Li, Ling [VerfasserIn]
• Verfasst von: Krämer, Bernhard [VerfasserIn]
• Verfasst von: Morgera, Stanislao [VerfasserIn]
• Verfasst von: Hocher, Berthold [VerfasserIn]
• Verfasst von: Tang, Donge [VerfasserIn]
• Verfasst von: Yin, Lianghong [VerfasserIn]
• Verfasst von: Dai, Yong [VerfasserIn]
• Titel: Integrated proteome and malonylome analyses reveal the neutrophil extracellular trap formation pathway in rheumatoid arthritis
• Verf.angabe: Biying Hu, Dandan Li, Zhipeng Zeng, Zeyu Zhang, Rui Cao, XiangNan Dong, Chen Yun, Ling Li, Bernhard Krämer, Stanislao Morgera, Berthold Hocher, Donge Tang, Lianghong Yin, Yong Dai
• E-Jahr: 2022
• Jahr: 30 June 2022
• Umfang: 10 S.
• Illustrationen: graphische Darstellungen
• Fussnoten: Online verfügbar: 27 April 2022, Artikelversion: 2 May 2022 ; Gesehen am 23.01.2024
• Titel Quelle: Enthalten in: Journal of proteomics
• Ort Quelle: New York, NY [u.a.] : Elsevier, 2008
• Jahr Quelle: 2022
• Band/Heft Quelle: 262(2022) vom: Juni, Artikel-ID 104597, Seite 1-10
• ISSN Quelle: 1876-7737
• DOI: doi:10.1016/j.jprot.2022.104597
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: Lysine malonylation
• Sach-SW: Mass spectrometry
• Sach-SW: Neutrophil extracellular trap formation
• Sach-SW: Posttranslational modifications
• Sach-SW: Rheumatoid arthritis
• K10plus-PPN: 1878676369

Abstract

Rheumatoid arthritis (RA) is an autoimmune inflammatory disease of unknown etiology in which the posttranslational modifications (PTMs) of proteins play an important role. PTMs, such as those involved in the formation of neutrophil extracellular traps (NETs), have been well studied. The excessive formation and release of NETs can mediate inflammation and joint destruction in RA. It has been gradually recognized that lysine malonylation (Kmal) can regulate some biological processes in some prokaryotes and eukaryotes. However, less is known about the role of Kmal in RA. We therefore performed proteome and malonylome analyses to explore the proteomic characteristics of the peripheral blood mononuclear cells from 36 RA patients and 82 healthy subjects. In total, 938 differentially expressed proteins (DEPs) and 42 differentially malonylated proteins (DMPs) with 55 Kmal sites were detected through a liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based analysis. Functional analysis showed that two DEPs with four malonylated sites and one DMP with a malonylated site were identified in the neutrophil extracellular trap formation (NETosis) pathway. Altogether, this study not only describes the characteristics of the malonylome in RA for the first time, but it also reveals that malonylation may be involved in the NETosis pathway. - Significance - This is the first report that reveals the proteomic features of Kmal in RA through a LC-MS/MS-based method. In this study, we found that several key DMPs were associated with the NETosis pathway, which contributes to the development of RA. The present results provide an informative dataset for the future exploration of Kmal in RA.