| |  Online-Ressource |
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| Verfasst von: | Denecke, Bernd [VerfasserIn]  |
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| | Wickert, Lucia [VerfasserIn] |
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| | Liu, Yan [VerfasserIn] |
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| | Ciuclan, Loredana [VerfasserIn] |
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| | Dooley, Steven [VerfasserIn] |
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| | Meindl-Beinker, Nadja M. [VerfasserIn] |
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| Titel: | Smad7 dependent expression signature highlights BMP2 and HK2 signaling in HSC transdifferentiation |
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| Verf.angabe: | Bernd Denecke, Lucia Wickert, Yan Liu, Loredana Ciuclan, Steven Dooley, Nadja M. Meindl-Beinker |
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| E-Jahr: | 2010 |
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| Jahr: | 7 November 2010 |
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| Umfang: | 14 S. |
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| Illustrationen: | Illustrationen |
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| Fussnoten: | Gesehen am 25.01.2024 |
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| Titel Quelle: | Enthalten in: World journal of gastroenterology |
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| Ort Quelle: | Beijing : WJG Press, 1995 |
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| Jahr Quelle: | 2010 |
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| Band/Heft Quelle: | 16(2010), 41 vom: Nov., Seite 5211-5224 |
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| ISSN Quelle: | 2219-2840 |
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| Abstract: | AIM: To analyse the influence of Smad7, antagonist of transforming growth factor (TGF)-β canonical signaling pathways on hepatic stellate cell (HSC) transdifferentiation in detail. - METHODS: We systematically analysed genes regulated by TGF-β/Smad7 in activated HSCs by microarray analysis and validated the results using real time polymerase chain reaction and Western blotting analysis. - RESULTS: We identified 100 known and unknown targets underlying the regulation of Smad7 expression and delineated 8 gene ontology groups. Hk2, involved in glycolysis, was one of the most downregulated proteins, while BMP2, activator of the Smad1/5/8 pathway, was extremely upregulated by Smad7. However, BMP2 dependent Smad1 activation could be inhibited in vitro by Smad7 overexpression in HSCs. - CONCLUSION: We conclude (1) the existence of a tight crosstalk of TGF-β and BMP2 pathways in HSCs and (2) a Smad7 dependently decreased sugar metabolism ameliorates HSC activation probably by energy withdrawal. |
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| DOI: | doi:10.3748/wjg.v16.i41.5211 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
kostenfrei: Volltext: https://doi.org/10.3748/wjg.v16.i41.5211 |
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| | DOI: https://doi.org/10.3748/wjg.v16.i41.5211 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | Female |
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| | Male |
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| | Animals |
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| | Molecular Sequence Data |
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| | Cells, Cultured |
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| | Signal Transduction |
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| | Rats |
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| | Gene Expression Regulation |
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| | Rats, Sprague-Dawley |
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| | Microarray Analysis |
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| | Rats, Wistar |
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| | Hepatic Stellate Cells |
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| | Smad7 Protein |
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| | Bone Morphogenetic Protein 2 |
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| | Cell Transdifferentiation |
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| | Tissue Kallikreins |
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| K10plus-PPN: | 1878999591 |
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| Verknüpfungen: | → Zeitschrift |
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Smad7 dependent expression signature highlights BMP2 and HK2 signaling in HSC transdifferentiation / Denecke, Bernd [VerfasserIn]; 7 November 2010 (Online-Ressource)
