| |  Online-Ressource |
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| Verfasst von: | Schmidt, Nora [VerfasserIn]  |
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| | Ganskih, Sabina [VerfasserIn] |
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| | Wei, Yuanjie [VerfasserIn] |
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| | Gabel, Alexander [VerfasserIn] |
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| | Zielinski, Sebastian [VerfasserIn] |
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| | Keshishian, Hasmik [VerfasserIn] |
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| | Lareau, Caleb A. [VerfasserIn] |
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| | Zimmermann, Liv [VerfasserIn] |
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| | Makroczyova, Jana [VerfasserIn] |
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| | Pearce, Cadence [VerfasserIn] |
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| | Krey, Karsten [VerfasserIn] |
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| | Hennig, Thomas [VerfasserIn] |
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| | Stegmaier, Sebastian [VerfasserIn] |
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| | Moyon, Lambert [VerfasserIn] |
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| | Horlacher, Marc [VerfasserIn] |
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| | Werner, Simone [VerfasserIn] |
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| | Aydin, Jens [VerfasserIn] |
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| | Olguin-Nava, Marco [VerfasserIn] |
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| | Potabattula, Ramya [VerfasserIn] |
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| | Kibe, Anuja [VerfasserIn] |
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| | Dölken, Lars [VerfasserIn] |
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| | Smyth, Redmond P. [VerfasserIn] |
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| | Caliskan, Neva [VerfasserIn] |
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| | Marsico, Annalisa [VerfasserIn] |
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| | Krempl, Christine [VerfasserIn] |
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| | Bodem, Jochen [VerfasserIn] |
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| | Pichlmair, Andreas [VerfasserIn] |
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| | Carr, Steven A. [VerfasserIn] |
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| | Chlanda, Petr [VerfasserIn] |
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| | Erhard, Florian [VerfasserIn] |
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| | Munschauer, Mathias [VerfasserIn] |
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| Titel: | SND1 binds SARS-CoV-2 negative-sense RNA and promotes viral RNA synthesis through NSP9 |
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| Verf.angabe: | Nora Schmidt, Sabina Ganskih, Yuanjie Wei, Alexander Gabel, Sebastian Zielinski, Hasmik Keshishian, Caleb A. Lareau, Liv Zimmermann, Jana Makroczyova, Cadence Pearce, Karsten Krey, Thomas Hennig, Sebastian Stegmaier, Lambert Moyon, Marc Horlacher, Simone Werner, Jens Aydin, Marco Olguin-Nava, Ramya Potabattula, Anuja Kibe, Lars Dölken, Redmond P. Smyth, Neva Caliskan, Annalisa Marsico, Christine Krempl, Jochen Bodem, Andreas Pichlmair, Steven A. Carr, Petr Chlanda, Florian Erhard, and Mathias Munschauer |
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| E-Jahr: | 2023 |
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| Jahr: | 26 October 2023 |
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| Umfang: | 40 S. |
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| Illustrationen: | Illustrationen |
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| Fussnoten: | Gesehen am 26.01.2024 ; Online verfügbar 3 October 2023, Version des Artikels 26 October 2023 |
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| Titel Quelle: | Enthalten in: Cell |
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| Ort Quelle: | [Cambridge, Mass.] : Cell Press, 1974 |
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| Jahr Quelle: | 2023 |
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| Band/Heft Quelle: | 186(2023), 22 vom: Okt., Seite 4834-4850, e1-e23 |
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| ISSN Quelle: | 1097-4172 |
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| Abstract: | Regulation of viral RNA biogenesis is fundamental to productive SARS-CoV-2 infection. To characterize host RNA-binding proteins (RBPs) involved in this process, we biochemically identified proteins bound to genomic and subgenomic SARS-CoV-2 RNAs. We find that the host protein SND1 binds the 5′ end of negative-sense viral RNA and is required for SARS-CoV-2 RNA synthesis. SND1-depleted cells form smaller replication organelles and display diminished virus growth kinetics. We discover that NSP9, a viral RBP and direct SND1 interaction partner, is covalently linked to the 5′ ends of positive- and negative-sense RNAs produced during infection. These linkages occur at replication-transcription initiation sites, consistent with NSP9 priming viral RNA synthesis. Mechanistically, SND1 remodels NSP9 occupancy and alters the covalent linkage of NSP9 to initiating nucleotides in viral RNA. Our findings implicate NSP9 in the initiation of SARS-CoV-2 RNA synthesis and unravel an unsuspected role of a cellular protein in orchestrating viral RNA production. |
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| DOI: | doi:10.1016/j.cell.2023.09.002 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1016/j.cell.2023.09.002 |
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| | Volltext: https://www.sciencedirect.com/science/article/pii/S0092867423009807 |
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| | DOI: https://doi.org/10.1016/j.cell.2023.09.002 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | host factors |
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| | omics technologies |
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| | proteomics |
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| | RNA binding proteins |
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| | RNA biology |
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| | RNA interactome |
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| | RNA virus |
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| | SARS-CoV-2 |
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| | systems biology |
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| | virus host interactions |
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| K10plus-PPN: | 1879047217 |
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| Verknüpfungen: | → Zeitschrift |
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SND1 binds SARS-CoV-2 negative-sense RNA and promotes viral RNA synthesis through NSP9 / Schmidt, Nora [VerfasserIn]; 26 October 2023 (Online-Ressource)
