| Online-Ressource |
Verfasst von: | Siegler, Benedikt Hermann [VerfasserIn]  |
| Thon, Jan Niklas [VerfasserIn]  |
| Altvater, Marc [VerfasserIn]  |
| Schenz, Judith [VerfasserIn]  |
| Larmann, Jan [VerfasserIn]  |
| Weigand, Markus A. [VerfasserIn]  |
| Weiterer, Sebastian [VerfasserIn]  |
Titel: | Abdominal surgery induces long-lasting changes in expression and binding of CTCF with impact on Major Histocompatibility Complex II transcription in circulating human monocytes |
Verf.angabe: | Benedikt Hermann Siegler, Jan Niklas Thon, Marc Altvater, Judith Schenz, Jan Larmann, Markus Alexander Weigand, Sebastian Weiterer |
E-Jahr: | 2023 |
Jahr: | October 25, 2023 |
Umfang: | 16 S. |
Illustrationen: | Diagramme |
Fussnoten: | Gesehen am 05.02.2024 |
Titel Quelle: | Enthalten in: PLOS ONE |
Ort Quelle: | San Francisco, California, US : PLOS, 2006 |
Jahr Quelle: | 2023 |
Band/Heft Quelle: | 18(2023), 10, Artikel-ID e0293347, Seite 1-16 |
ISSN Quelle: | 1932-6203 |
Abstract: | BackgroundPostoperative immunosuppression has been recognized as an important driver of surgery-related morbidity and mortality. It is characterized by lymphocyte depression and impaired monocyte capability to present foreign antigens to T-cells via Major Histocompatibility Complex, Class II (MHC-II) molecules. In patients with postoperative abdominal sepsis, we previously detected a persisting differential binding of the CCCTC-Binding Factor (CTCF), a superordinate regulator of transcription, inside the MHC-II region with specific impact on human leucocyte antigen (HLA) gene expression. In this prospective exploratory study, we investigated to which extent major surgery affects the MHC-II region of circulating CD14+-monocytes.ResultsIn non-immunocompromised patients undergoing elective major abdominal surgery, a postoperative loss of monocyte HLA-DR surface receptor density was accompanied by a decline in the transcription levels of the classical MHC-II genes HLA-DRA, HLA-DRB1, HLA-DPA1 and HLA-DPB1. The surgical event decreased the expression of the transcriptional MHC-II regulators CIITA and CTCF and led to a lower CTCF enrichment at an intergenic sequence within the HLA-DR subregion. During the observation period, we found a slow and only incomplete restoration of monocyte HLA-DR surface receptor density as well as a partial recovery of CIITA, HLA-DRA and HLA-DRB1 expression. In contrast, transcription of HLA-DPA1, HLA-DPB1, CTCF and binding of CTCF within the MHC-II remained altered.ConclusionIn circulating monocytes, major surgery does not globally affect MHC-II transcription but rather induces specific changes in the expression of selected HLA genes, followed by differential recovery patterns and accompanied by a prolonged reduction of CTCF expression and binding within the MHC-II region. Our results hint toward a long-lasting impact of a major surgical intervention on monocyte functionality, possibly mediated by epigenetic changes that endure the life span of the individual cell. |
DOI: | doi:10.1371/journal.pone.0293347 |
URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
kostenfrei: Volltext: https://doi.org/10.1371/journal.pone.0293347 |
| kostenfrei: Volltext: https://www.webofscience.com/api/gateway?GWVersion=2&SrcAuth=DOISource&SrcApp=WOS&KeyAID=10.1371%2Fjournal.pone.0293347& ... |
| DOI: https://doi.org/10.1371/journal.pone.0293347 |
Datenträger: | Online-Ressource |
Sprache: | eng |
Sach-SW: | CHOLECYSTECTOMY |
| GENES |
| RECEPTORS |
| SERUM INTERLEUKIN-6 |
K10plus-PPN: | 1879951517 |
Verknüpfungen: | → Zeitschrift |
Abdominal surgery induces long-lasting changes in expression and binding of CTCF with impact on Major Histocompatibility Complex II transcription in circulating human monocytes / Siegler, Benedikt Hermann [VerfasserIn]; October 25, 2023 (Online-Ressource)