An engineered β-hairpin peptide forming thermostable complexes with ZnII, NiII, and CuII through a His3 site

• Verfasst von: Pham, Truc Lam [VerfasserIn]
• Verfasst von: Fazliev, Sunnatullo [VerfasserIn]
• Verfasst von: Baur, Philipp [VerfasserIn]
• Verfasst von: Comba, Peter [VerfasserIn]
• Verfasst von: Thomas, Franziska [VerfasserIn]
• Titel: An engineered β-hairpin peptide forming thermostable complexes with ZnII, NiII, and CuII through a His3 site
• Verf.angabe: Truc Lam Pham, Sunnatullo Fazliev, Philipp Baur, Peter Comba, and Franziska Thomas
• E-Jahr: 2023
• Jahr: February 1, 2023
• Umfang: 6 S.
• Fussnoten: Gesehen am 19.02.2024
• Titel Quelle: Enthalten in: ChemBioChem
• Ort Quelle: Weinheim : Wiley-VCH, 2000
• Jahr Quelle: 2023
• Band/Heft Quelle: 24(2023), 3, Artikel-ID e202200588, Seite 1-6
• ISSN Quelle: 1439-7633
• DOI: doi:10.1002/cbic.202200588
• Datenträger: Online-Ressource
• Sprache: eng
• Bibliogr. Hinweis: Forschungsdaten: Pham, Truc Lam: An engineered β-hairpin peptide forming thermostable complexes with ZnII, NiII, and CuII through a His3 site [research data]
• Sach-SW: bioinorganic chemistry
• Sach-SW: extremophiles
• Sach-SW: protein engineering
• Sach-SW: protein folding
• Sach-SW: tryptophan zippers
• K10plus-PPN: 1881152952

Abstract

The three-dimensional structure of a peptide, which determines its function, can denature at elevated temperatures, in the presence of chaotropic reagents, or in organic solvents. These factors limit the applicability of peptides. Herein, we present an engineered β-hairpin peptide containing a His3 site that forms complexes with ZnII, NiII, and CuII. Circular dichroism spectroscopy shows that the peptide−metal complexes exhibit melting temperatures up to 80 °C and remain folded in 6 M guanidine hydrochloride as well as in organic solvents. Intrinsic fluorescence titration experiments were used to determine the dissociation constants of metal binding in the nano- to sub-nanomolar range. The coordination geometry of the peptide−CuII complex was studied by EPR spectroscopy, and a distorted square planar coordination geometry with weak interactions to axial ligands was revealed. Due to their impressive stability, the presented peptide−metal complexes open up interesting fields of application, such as the development of a new class of peptide−metal catalysts for stereoselective organic synthesis or the directed design of extremophilic β-sheet peptides.