Multimaterial 3D laser printing of cell-adhesive and cell-repellent hydrogels [data]

• Verfasst von: Schwegler, Niklas [VerfasserIn]
• Verfasst von: Gebert, Tanisha [VerfasserIn]
• Verfasst von: Villiou, Maria [VerfasserIn]
• Verfasst von: Colombo, Federico [VerfasserIn]
• Verfasst von: Schamberger, Barbara [VerfasserIn]
• Verfasst von: Selhuber-Unkel, Christine [VerfasserIn]
• Verfasst von: Thomas, Franziska [VerfasserIn]
• Verfasst von: Blasco, Eva [VerfasserIn]
• Titel: Multimaterial 3D laser printing of cell-adhesive and cell-repellent hydrogels [data]
• Verf.angabe: Niklas Schwegler, Tanisha Gebert, Maria Villiou, Federico Colombo, Barbara Schamberger, Christine Selhuber-Unkel, Franziska Thomas, Eva Blasco
• Verlagsort: Heidelberg
• Verlag: Universität
• E-Jahr: 2024
• Jahr: 2024-02-16
• Umfang: 1 Online-Ressource (2 Files)
• Fussnoten: Finanziert durch: Spotlight Project “Synthetic Immunology” of the Flagship Initiative Engineering Molecular Systems (FI EMS; funded by the BMBF and the Ministry of Science Baden-Württemberg); Excellence Cluster “3D Matter Made to Order” (funded by the DFG): EXC 2082/1 390761711; ERC Consolidator Grant PHOTOMECH: Grant no. 101001797; Volkswagen Foundation Initiative “Life?,”: Az. 96733 ; Gesehen am 21.02.2024
• DOI: doi:10.11588/data/V2XXS8
• Datenträger: Online-Ressource
• Dokumenttyp: Forschungsdaten
• Dokumenttyp: Datenbank
• Sprache: eng
• Bibliogr. Hinweis: Forschungsdaten zu: Schwegler, Niklas, 1998 - : Multimaterial 3D laser printing of cell-adhesive and cell-repellent hydrogels
• Sonstige Nr.: Grant number: DFG EXC 2082/1 390761711
• Sonstige Nr.: Grant number: ERC Consolidator Grant PHOTOMECH 101001797
• Sonstige Nr.: Grant number: Volkswagen Foundation Initiative “Life?” 96733
• Sach-SW: Chemistry
• K10plus-PPN: 1881329143
• K10plus-PPN:
  Universitätsbibliothek
• Lokale URL UB: Zum Volltext

Abstract

This study introduces a straightforward method for manufacturing 3D microstructured cell-adhesive and cell-repellent multimaterials using two-photon laser printing. Compared to existing strategies, this approach offers bottom-up molecular control, high customizability and rapid and precise 3D fabrication. The printable cell-adhesive PEG-based material includes an RGD-containing peptide synthesized through solid-phase peptide synthesis, allowing for precise control of the peptide design. Remarkably, minimal amounts of RGD peptide (< 0.1 wt%) suffice for imparting cell-adhesiveness, while maintaining identical mechanical properties in the 3D printed microstructures to those of the cell-repellent, PEG-based material. Fluorescent labeling of the RGD peptide facilitates visualization of its presence in cell-adhesive areas. To demonstrate the broad applicability of our system, we showcase the fabrication of cell-adhesive 2.5D and 3D structures, fostering the adhesion of fibroblast cells within these architectures. Thus, this approach allows for the printing of high-resolution, true 3D structures suitable for diverse applications, including cellular studies in complex environments.