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| Verfasst von: | Medert, Rebekka [VerfasserIn]  |
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| | Thumberger, Thomas [VerfasserIn] |
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| | Tavhelidse-Suck, Tinatini [VerfasserIn] |
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| | Hub, Tobias [VerfasserIn] |
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| | Kellner, Tanja [VerfasserIn] |
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| | Oguchi, Yoko [VerfasserIn] |
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| | Dlugosz, Sascha [VerfasserIn] |
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| | Zimmermann, Frank [VerfasserIn] |
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| | Wittbrodt, Joachim [VerfasserIn] |
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| | Freichel, Marc [VerfasserIn] |
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| Titel: | Efficient single copy integration via homology-directed repair (scHDR) by 5′modification of large DNA donor fragments in mice |
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| Verf.angabe: | Rebekka Medert, Thomas Thumberger, Tinatini Tavhelidse-Suck, Tobias Hub, Tanja Kellner, Yoko Oguchi, Sascha Dlugosz, Frank Zimmermann, Joachim Wittbrodt and Marc Freichel |
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| E-Jahr: | 2023 |
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| Jahr: | 22 February 2023 |
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| Umfang: | 14 S. |
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| Illustrationen: | Illustrationen |
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| Fussnoten: | Online veröffentlicht: 19. Dezember 2022 ; Gesehen am 26.02.2024 |
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| Titel Quelle: | Enthalten in: Nucleic acids research |
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| Ort Quelle: | Oxford : Oxford Univ. Press, 1974 |
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| Band/Heft Quelle: | 51(2023), 3, Artikel-ID e14, Seite 1-14 |
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| ISSN Quelle: | 1362-4962 |
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| Abstract: | CRISPR/Cas-based approaches have largely replaced conventional gene targeting strategies. However, homology-directed repair (HDR) in the mouse genome is not very efficient, and precisely inserting longer sequences using HDR remains challenging given that donor constructs preferentially integrate as concatemers. Here, we showed that injecting 5′ biotinylated donor DNA into mouse embryos at the two-cell stage led to efficient single-copy HDR (scHDR) allele generation. Our dedicated genotyping strategy showed that these alleles occurred with frequencies of 19%, 20%, and 26% at three independent gene loci, indicating that scHDR was dramatically increased by 5′ biotinylation. Thus, we suggest that the combination of a 5′ biotinylated donor and diligent analysis of concatemer integration are prerequisites for efficiently and reliably generating conditional alleles or other large fragment knock-ins in the mouse genome. |
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| DOI: | doi:10.1093/nar/gkac1150 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1093/nar/gkac1150 |
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| | DOI: https://doi.org/10.1093/nar/gkac1150 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| K10plus-PPN: | 1881631907 |
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| Verknüpfungen: | → Zeitschrift |
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Efficient single copy integration via homology-directed repair (scHDR) by 5′modification of large DNA donor fragments in mice / Medert, Rebekka [VerfasserIn]; 22 February 2023 (Online-Ressource)
