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| Verfasst von: | Yousuf, Suhail [VerfasserIn]  |
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| | Qiu, Mengjie [VerfasserIn] |
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| | Voith von Voithenberg, Lena [VerfasserIn] |
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| | Hulkkonen, Johannes [VerfasserIn] |
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| | Macinkovic, Igor [VerfasserIn] |
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| | Schulz, Axel R. [VerfasserIn] |
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| | Hartmann, Domenic [VerfasserIn] |
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| | Müller, Florian [VerfasserIn] |
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| | Mijatovic, Margarete [VerfasserIn] |
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| | Ibberson, David [VerfasserIn] |
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| | Al Halabi, Karam [VerfasserIn] |
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| | Hetzer, Jenny [VerfasserIn] |
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| | Anders, Simon [VerfasserIn] |
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| | Brüne, Bernhard [VerfasserIn] |
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| | Mei, Henrik E. [VerfasserIn] |
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| | Imbusch, Charles [VerfasserIn] |
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| | Brors, Benedikt [VerfasserIn] |
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| | Heikenwälder, Mathias [VerfasserIn] |
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| | Gaida, Matthias [VerfasserIn] |
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| | Büchler, Markus W. [VerfasserIn] |
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| | Weigert, Andreas [VerfasserIn] |
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| | Hackert, Thilo [VerfasserIn] |
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| | Roth, Susanne [VerfasserIn] |
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| Titel: | Spatially resolved multi-omics single-cell analyses inform mechanisms of immune dysfunction in pancreatic cancer |
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| Verf.angabe: | Suhail Yousuf, Mengjie Qiu, Lena Voith von Voithenberg, Johannes Hulkkonen, Igor Macinkovic, Axel R. Schulz, Domenic Hartmann, Florian Mueller, Margarete Mijatovic, David Ibberson, Karam T. AlHalabi, Jenny Hetzer, Simon Anders, Bernhard Brüne, Henrik E. Mei, Charles D. Imbusch, Benedikt Brors, Mathias Heikenwälder, Matthias M. Gaida, Markus W. Büchler, Andreas Weigert, Thilo Hackert, Susanne Roth |
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| E-Jahr: | 2023 |
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| Jahr: | October 2023 |
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| Umfang: | 32 S. |
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| Fussnoten: | Gesehen am 27.02.2024 |
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| Titel Quelle: | Enthalten in: Gastroenterology |
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| Ort Quelle: | New York, NY : Elsevier, 1949 |
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| Band/Heft Quelle: | 165(2023), 4 vom: Okt., Seite 891-908, e1-e14 |
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| ISSN Quelle: | 1528-0012 |
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| Abstract: | Background & Aims - As pancreatic ductal adenocarcinoma (PDAC) continues to be recalcitrant to therapeutic interventions, including poor response to immunotherapy, albeit effective in other solid malignancies, a more nuanced understanding of the immune microenvironment in PDAC is urgently needed. We aimed to unveil a detailed view of the immune micromilieu in PDAC using a spatially resolved multimodal single-cell approach. - Methods - We applied single-cell RNA sequencing, spatial transcriptomics, multiplex immunohistochemistry, and mass cytometry to profile the immune compartment in treatment-naïve PDAC tumors and matched adjacent normal pancreatic tissue, as well as in the systemic circulation. We determined prognostic associations of immune signatures and performed a meta-analysis of the immune microenvironment in PDAC and lung adenocarcinoma on single-cell level. - Results - We provided a spatially resolved fine map of the immune landscape in PDAC. We substantiated the exhausted phenotype of CD8 T cells and immunosuppressive features of myeloid cells, and highlighted immune subsets with potentially underappreciated roles in PDAC that diverged from immune populations within adjacent normal areas, particularly CD4 T cell subsets and natural killer T cells that are terminally exhausted and acquire a regulatory phenotype. Differential analysis of immune phenotypes in PDAC and lung adenocarcinoma revealed the presence of extraordinarily immunosuppressive subtypes in PDAC, along with a distinctive immune checkpoint composition. - Conclusions - Our study sheds light on the multilayered immune dysfunction in PDAC and presents a holistic view of the immune landscape in PDAC and lung adenocarcinoma, providing a comprehensive resource for functional studies and the exploration of therapeutically actionable targets in PDAC. |
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| DOI: | doi:10.1053/j.gastro.2023.05.036 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
kostenfrei: Volltext: https://doi.org/10.1053/j.gastro.2023.05.036 |
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| | kostenfrei: Volltext: https://www.sciencedirect.com/science/article/pii/S0016508523008107 |
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| | DOI: https://doi.org/10.1053/j.gastro.2023.05.036 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | Lung Cancer |
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| | Pancreatic Cancer |
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| | Single-Cell Multi-Omics |
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| | Tumor Immunology |
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| K10plus-PPN: | 1881718107 |
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| Verknüpfungen: | → Zeitschrift |
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Spatially resolved multi-omics single-cell analyses inform mechanisms of immune dysfunction in pancreatic cancer / Yousuf, Suhail [VerfasserIn]; October 2023 (Online-Ressource)
