A Practical Guide to Metabolomics Applications in Health and Disease

• Titel: A Practical Guide to Metabolomics Applications in Health and Disease
• Titelzusatz: From Samples to Insights into Metabolism
• Mitwirkende: Ivanisevic, Julijana [HerausgeberIn]
• Mitwirkende: Giera, Martin [HerausgeberIn]
• Verf.angabe: edited by Julijana Ivanisevic, Martin Giera
• Ausgabe: 1st ed. 2023.
• Verlagsort: Cham
• Verlagsort: Cham
• Verlag: Springer International Publishing
• Verlag: Imprint: Springer
• E-Jahr: 2023
• Jahr: 2023.
• Jahr: 2023.
• Umfang: 1 Online-Ressource(IX, 453 p.)
• Gesamttitel/Reihe: Learning Materials in Biosciences
• ISBN: 978-3-031-44256-8
• DOI: doi:10.1007/978-3-031-44256-8
• Datenträger: Online-Ressource
• Sprache: eng
• Bibliogr. Hinweis: Erscheint auch als : Druck-Ausgabe
• Bibliogr. Hinweis: Erscheint auch als : Druck-Ausgabe
• Bibliogr. Hinweis: Erscheint auch als : Druck-Ausgabe
• K10plus-PPN: 1882479327
• K10plus-PPN:
  Universitätsbibliothek
• Lokale URL UB: Zum Volltext
• Lokale URL UB: Bibliothek der Medizinischen Fakultät Mannheim der Universität Heidelberg
• Bibliothek/Idn: UW / m4495732102
• Lokale URL Inst.: Zum Volltext

Abstract

Part 1. Introduction -- 1. Introduction -- Part 2. From Sample Collection to Data Acquisition 2. Sample collection and handling: practices to follow -- 3. How to prepare your samples for polar metabolite analysis? -- 4. How to extract lipid metabolites and perform lipid analysis? -- Part 3. From Data Processing to Polar and Lipid Metabolite Identification 5. Data processing & metabolite annotation using XCMS-METLIN -- 6. GC/MS data processing and metabolite identification -- Part 4. From Statistical Data Analysis to Insights into Biology 7. Workflow for knowledge discovery from metabolomic data using chemometrics -- 8. Using Quantitative Metabolomics and Data Enrichment to Interpret the Biochemistry of a Novel Disease -- Part 5. Metabolomics to Decipher Physiology in Health and Disease: Clinical Research Studies 9. Automated sample preparation for blood plasma lipidomics -- 10. NMR based Metabolomics in the context of exercise -- 11. Identifying sex-specific cancer metabolites and associations to prognosis -- 12. A lipidome-wide association study: Data processing, annotation and analysis workflow using MS-DIAL and R -- 13. Quantitative analysis of eicosanoids and other oxylipins – Investigation of oxidative stress and inflammation by means of targeted metabolomics of oxylipins in cell culture -- Part 6. Spatial Metabolite Analysis using MS-assisted Tissue Imaging 14. Spatial lipidomics on and off-tissue analysis -- 15. Spatial isotopic tracing -- 16. Quantitative imaging using SIMS.

Abstract

This well-accessible textbook constitutes a practical guide to metabolomics approaches and applications in an integrative manner. Illustrated by numerous real-life examples and case studies the book teaches how to set up and carry out metabolomics experiments, from sample collection to storage techniques, and guides the reader through the potentials and challenges of metabolite analysis. In addition, this textbook shows how to obtain insights into molecular mechanisms deployed in different physiological contexts (e.g., exercise, healthy ageing) as well as in different (metabolic) diseases, be it Diabetes, Cancer, Alzheimer´s Disease, Asthma or others. The book brings together knowledge from biochemical, biological, and medical aspects, and further equips the reader with know-how from sample collection and handling to data processing, analysis and interpretation. This textbook is a must-have for advanced undergraduates and graduate students interested in applied biochemistry and metabolomics, as well as for instructors who teach courses in biomedicine, physiology or biochemistry.