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Verfasst von:Jagirdar, Rajesh M. [VerfasserIn]   i
 Pitaraki, Eleanna [VerfasserIn]   i
 Rouka, Erasmia [VerfasserIn]   i
 Papazoglou, Eleftherios D. [VerfasserIn]   i
 Bartosova, Maria [VerfasserIn]   i
 Zebekakis, Pantelis [VerfasserIn]   i
 Schmitt, Claus P. [VerfasserIn]   i
 Zarogiannis, Sotirios G. [VerfasserIn]   i
 Liakopoulos, Vassilios [VerfasserIn]   i
Titel:Differential effects of biocompatible peritoneal dialysis fluids on human mesothelial and endothelial cells in 2D and 3D phenotypes
Verf.angabe:Rajesh M. Jagirdar, Eleanna Pitaraki, Erasmia Rouka, Eleftherios D. Papazoglou, Maria Bartosova, Pantelis Zebekakis, Claus Peter Schmitt, Sotirios G. Zarogiannis, Vassilios Liakopoulos
E-Jahr:2024
Jahr:May 2024
Umfang:11 S.
Illustrationen:Illustrationen
Fussnoten:Gesehen am 11.03.2024
Titel Quelle:Enthalten in: Artificial organs
Ort Quelle:Oxford [u.a.] : Wiley-Blackwell, 1977
Jahr Quelle:2024
Band/Heft Quelle:48(2024), 5 vom: Mai, Seite 484-494
ISSN Quelle:1525-1594
Abstract:Introduction Peritoneal dialysis (PD) is a life maintaining treatment in patients with end-stage renal disease. Its chronic application leads to peritoneal mesothelial layer denudation and fibrotic transformation along with vascular activation of inflammatory pathways. The impact of different PD fluids (PDF) on mesothelial and endothelial cell function and repair mechanisms are not comprehensively described. Materials and Methods Mesothelial (MeT-5A) and endothelial cells (EA.hy926) were cultured in 1:1 ratio with cell medium and different PDF (icodextrin-based, amino acid-based, and glucose-based). Cell adhesion, cell migration, and cell proliferation in 2D and spheroid formation and collagen gel contraction assays in 3D cell cultures were performed. Results Cell proliferation and cell-mediated gel contraction were both significantly decreased in all conditions. 3D spheroid formation was significantly reduced with icodextrin and amino acid PDF, but unchanged with glucose PDF. Adhesion was significantly increased by amino acid PDF in mesothelial cells and decreased by icodextrin and amino acid PDF in endothelial cells. Migration capacity was significantly decreased in mesothelial cells by all three PDF, while endothelial cells remained unaffected. Conclusions In 3D phenotypes the effects of PDF are more uniform in both mesothelial and endothelial cells, mitigating spheroid formation and gel contraction. On the contrary, effects on 2D phenotypes are more uniform in the icodextrin and amino acid PDF as opposed to glucose ones and affect mesothelial cells more variably. 2D and 3D comparative assessments of PDF effects on the main peritoneal membrane cell barriers, the mesothelial and endothelial, could provide useful translational information for PD studies.
DOI:doi:10.1111/aor.14703
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext: https://doi.org/10.1111/aor.14703
 Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1111/aor.14703
 DOI: https://doi.org/10.1111/aor.14703
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:3D cell cultures
 endothelial cells
 endothelial-to-mesenchymal transition
 mesothelial cells
 peritoneal dialysis
 peritoneal dialysis fluids
K10plus-PPN:1883076056
Verknüpfungen:→ Zeitschrift

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