Osteopontin but not osteonectin favors the metastatic growth of pancreatic cancer cell lines

• Verfasst von: Zhivkova-Galunska, Maria [VerfasserIn]
• Verfasst von: Adwan, Hassan [VerfasserIn]
• Verfasst von: Eyol, Ergül [VerfasserIn]
• Verfasst von: Kleeff, Jörg H. [VerfasserIn]
• Verfasst von: Kolb, Armin [VerfasserIn]
• Verfasst von: Bergmann, Frank [VerfasserIn]
• Verfasst von: Berger, Martin R. [VerfasserIn]
• Titel: Osteopontin but not osteonectin favors the metastatic growth of pancreatic cancer cell lines
• Verf.angabe: Maria Zhivkova-Galunska, Hassan Adwan, Ergül Eyol, Jörg Kleeff, Armin Kolb, Frank Bergmann, Martin R. Berger
• Jahr: 2010
• Umfang: 11 S.
• Fussnoten: Gesehen am 12.03.2024
• Titel Quelle: Enthalten in: Cancer biology & therapy
• Ort Quelle: London : Informa UK Limited, Taylor & Francis Group, 2002
• Jahr Quelle: 2010
• Band/Heft Quelle: 10(2010), 1, Seite 54-64
• ISSN Quelle: 1555-8576
• DOI: doi:10.4161/cbt.10.1.12161
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1883190983

Abstract

Pancreatic ductal adenocarcinoma (PDAC) is the fourth leading cause of cancer- related death in Western countries and among the malignancies with the worst prognosis. Osteonectin and osteopontin, two proteins of the extracellular matrix, have been found to be up-regulated in PDAC. In the present study the expression of osteopontin mRNA as determined in a panel of 14 hu­man pancreatic cancer cell lines was significantly related to the growth these cell lines in the liver of nude rats (p=0.001) whereas osteonectin showed a trend of being negatively related to pancreatic cancer cell growth in vivo (p=0.10). In an in vitro co-culture model of human Suit2-007 and rat AsML PDAC cells with rat hepatocytes, a clearly increased expression of OPN mRNA was found in the tumor cells. In addition both, down-regulation of osteopontin with specific antisense oligonucleotides and treatment with exogenous rh-osteonectin were associated with reduced cell proliferation. In accordance with the latter finding down-regula­tion of osteonectin was coupled with increased proliferation. This evidence supports a protu­morigenic role of osteopontin and points to an anti-tumorigenic role of osteonectin in PDAC.