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| Verfasst von: | Jakob, Sara [VerfasserIn]  |
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| | Steinchen, Wieland [VerfasserIn] |
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| | Hanßmann, Juri [VerfasserIn] |
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| | Rosum, Julia [VerfasserIn] |
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| | Langenfeld, Katja [VerfasserIn] |
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| | Osorio Valeriano, Manuel [VerfasserIn] |
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| | Steube, Niklas [VerfasserIn] |
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| | Giammarinaro, Pietro Ivan [VerfasserIn] |
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| | Hochberg, Georg K. A. [VerfasserIn] |
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| | Glatter, Timo [VerfasserIn] |
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| | Bange, Gert [VerfasserIn] |
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| | Diepold, Andreas [VerfasserIn] |
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| | Thanbichler, Martin Rudolf [VerfasserIn] |
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| Titel: | The virulence regulator VirB from Shigella flexneri uses a CTP-dependent switch mechanism to activate gene expression |
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| Verf.angabe: | Sara Jakob, Wieland Steinchen, Juri Hanßmann, Julia Rosum, Katja Langenfeld, Manuel Osorio-Valeriano, Niklas Steube, Pietro I. Giammarinaro, Georg K.A. Hochberg, Timo Glatter, Gert Bange, Andreas Diepold & Martin Thanbichler |
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| E-Jahr: | 2024 |
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| Jahr: | 05 January 2024 |
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| Umfang: | 18 S. |
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| Illustrationen: | Illustrationen |
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| Fussnoten: | Gesehen am 14.03.2024 |
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| Titel Quelle: | Enthalten in: Nature Communications |
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| Ort Quelle: | [London] : Springer Nature, 2010 |
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| Jahr Quelle: | 2024 |
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| Band/Heft Quelle: | 15(2024), Artikel-ID 318, Seite 1-18 |
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| ISSN Quelle: | 2041-1723 |
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| Abstract: | The transcriptional antisilencer VirB acts as a master regulator of virulence gene expression in the human pathogen Shigella flexneri. It binds DNA sequences (virS) upstream of VirB-dependent promoters and counteracts their silencing by the nucleoid-organizing protein H-NS. However, its precise mode of action remains unclear. Notably, VirB is not a classical transcription factor but related to ParB-type DNA-partitioning proteins, which have recently been recognized as DNA-sliding clamps using CTP binding and hydrolysis to control their DNA entry gate. Here, we show that VirB binds CTP, embraces DNA in a clamp-like fashion upon its CTP-dependent loading at virS sites and slides laterally on DNA after clamp closure. Mutations that prevent CTP-binding block VirB loading in vitro and abolish the formation of VirB nucleoprotein complexes as well as virulence gene expression in vivo. Thus, VirB represents a CTP-dependent molecular switch that uses a loading-and-sliding mechanism to control transcription during bacterial pathogenesis. |
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| DOI: | doi:10.1038/s41467-023-44509-z |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
kostenfrei: Volltext: https://doi.org/10.1038/s41467-023-44509-z |
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| | kostenfrei: Volltext: https://www.nature.com/articles/s41467-023-44509-z |
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| | DOI: https://doi.org/10.1038/s41467-023-44509-z |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | Bacterial development |
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| | Bacterial pathogenesis |
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| | Transcription |
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| K10plus-PPN: | 1883443369 |
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| Verknüpfungen: | → Zeitschrift |
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¬The¬ virulence regulator VirB from Shigella flexneri uses a CTP-dependent switch mechanism to activate gene expression / Jakob, Sara [VerfasserIn]; 05 January 2024 (Online-Ressource)
