| Online-Ressource |
Verfasst von: | Jakob, Sara [VerfasserIn]  |
| Steinchen, Wieland [VerfasserIn]  |
| Hanßmann, Juri [VerfasserIn]  |
| Rosum, Julia [VerfasserIn]  |
| Langenfeld, Katja [VerfasserIn]  |
| Osorio Valeriano, Manuel [VerfasserIn]  |
| Steube, Niklas [VerfasserIn]  |
| Giammarinaro, Pietro Ivan [VerfasserIn]  |
| Hochberg, Georg K. A. [VerfasserIn]  |
| Glatter, Timo [VerfasserIn]  |
| Bange, Gert [VerfasserIn]  |
| Diepold, Andreas [VerfasserIn]  |
| Thanbichler, Martin Rudolf [VerfasserIn]  |
Titel: | The virulence regulator VirB from Shigella flexneri uses a CTP-dependent switch mechanism to activate gene expression |
Verf.angabe: | Sara Jakob, Wieland Steinchen, Juri Hanßmann, Julia Rosum, Katja Langenfeld, Manuel Osorio-Valeriano, Niklas Steube, Pietro I. Giammarinaro, Georg K.A. Hochberg, Timo Glatter, Gert Bange, Andreas Diepold & Martin Thanbichler |
E-Jahr: | 2024 |
Jahr: | 05 January 2024 |
Umfang: | 18 S. |
Illustrationen: | Illustrationen |
Fussnoten: | Gesehen am 14.03.2024 |
Titel Quelle: | Enthalten in: Nature Communications |
Ort Quelle: | [London] : Springer Nature, 2010 |
Jahr Quelle: | 2024 |
Band/Heft Quelle: | 15(2024), Artikel-ID 318, Seite 1-18 |
ISSN Quelle: | 2041-1723 |
Abstract: | The transcriptional antisilencer VirB acts as a master regulator of virulence gene expression in the human pathogen Shigella flexneri. It binds DNA sequences (virS) upstream of VirB-dependent promoters and counteracts their silencing by the nucleoid-organizing protein H-NS. However, its precise mode of action remains unclear. Notably, VirB is not a classical transcription factor but related to ParB-type DNA-partitioning proteins, which have recently been recognized as DNA-sliding clamps using CTP binding and hydrolysis to control their DNA entry gate. Here, we show that VirB binds CTP, embraces DNA in a clamp-like fashion upon its CTP-dependent loading at virS sites and slides laterally on DNA after clamp closure. Mutations that prevent CTP-binding block VirB loading in vitro and abolish the formation of VirB nucleoprotein complexes as well as virulence gene expression in vivo. Thus, VirB represents a CTP-dependent molecular switch that uses a loading-and-sliding mechanism to control transcription during bacterial pathogenesis. |
DOI: | doi:10.1038/s41467-023-44509-z |
URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
kostenfrei: Volltext: https://doi.org/10.1038/s41467-023-44509-z |
| kostenfrei: Volltext: https://www.nature.com/articles/s41467-023-44509-z |
| DOI: https://doi.org/10.1038/s41467-023-44509-z |
Datenträger: | Online-Ressource |
Sprache: | eng |
Sach-SW: | Bacterial development |
| Bacterial pathogenesis |
| Transcription |
K10plus-PPN: | 1883443369 |
Verknüpfungen: | → Zeitschrift |
¬The¬ virulence regulator VirB from Shigella flexneri uses a CTP-dependent switch mechanism to activate gene expression / Jakob, Sara [VerfasserIn]; 05 January 2024 (Online-Ressource)