Effect-directed analysis of Ah receptor-mediated activities caused by PAHs in suspended particulate matter sampled in flood events

• Verfasst von: Wölz, Jan [VerfasserIn]
• Verfasst von: Brack, W. [VerfasserIn]
• Verfasst von: Moehlenkamp, C. [VerfasserIn]
• Verfasst von: Claus, E. [VerfasserIn]
• Verfasst von: Braunbeck, Thomas [VerfasserIn]
• Verfasst von: Hollert, Henner [VerfasserIn]
• Titel: Effect-directed analysis of Ah receptor-mediated activities caused by PAHs in suspended particulate matter sampled in flood events
• Verf.angabe: J. Wölz, W. Brack, C. Moehlenkamp, E. Claus, Th. Braunbeck, H. Hollert
• E-Jahr: 2010
• Jahr: 15 July 2010
• Umfang: 7 S.
• Illustrationen: Diagramme
• Fussnoten: Gesehen am 15.03.2024
• Titel Quelle: Enthalten in: The science of the total environment
• Ort Quelle: Amsterdam [u.a.] : Elsevier Science, 1972
• Jahr Quelle: 2010
• Band/Heft Quelle: 408(2010), 16 vom: Juli, Seite 3327-3333
• ISSN Quelle: 1879-1026
• DOI: doi:10.1016/j.scitotenv.2010.03.029
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: Ah receptor
• Sach-SW: Dioxin-like activity
• Sach-SW: Effect-directed analysis
• Sach-SW: Flood
• Sach-SW: Fractionation
• Sach-SW: PAH
• Sach-SW: Suspended particulate matter
• K10plus-PPN: 1883605083

Abstract

Suspended particulate matter (SPM) sampled during a flood event in the year 2004 at the rivers Neckar and Rhine (Southwest Germany) was assessed for aryl hydrocarbon receptor (AhR)-mediated activities using EROD induction in the rainbow trout liver cell line RTL-W1. All EROD inductions were normalized to the positive control TCDD and given as bio-TEQ values. Since all samples indicated elevated AhR-mediated toxicities, an effect-directed analysis (EDA) was applied to identify substances causing the effects. In three primary fractions (F1 to F3) non-polar aliphatics, non-polar aromatic substances and more polar substances were separated. Fraction F2, co-eluting with non-polar polyaromatic substances (PACs) including polycyclic aromatic hydrocarbons (PAHs) gave highest AhR-agonistic effects and, thus, were sub-fractionated into seven secondary fractions (F2-1 to F2-7). Fraction F2-1, co-eluting with PCBs and PCDD/Fs, did not cause AhR-agonist activities. F2-2 to F2-4 containing PACs of less than 16 aromatic C-atoms produced minor activities. Highest inductions were detected with fraction F2-5 to F2-7, containing substances of more than 16 aromatic C-atoms (bio-TEQs up to approximately 4500pg/g). Concentrations and relative potencies (REPs) of priority EPA-PAHs allowed the calculation of chemical toxicity equivalent concentrations (chem-TEQ values). Based on the chem-TEQs, EPA-PAHs explained between 5 and 58% of crude extract bio-TEQs from both rivers. Whereas fractions F2-1 to F2-4 indicated no biological activities, EPA-PAHs in fraction F2-5 to F2-7 accounted for 2 to 137% of AhR-related activities.