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Verfasst von:Wohnsland, Sascha [VerfasserIn]   i
 Bürgers, Heinrich [VerfasserIn]   i
 Kuschinsky, Wolfgang [VerfasserIn]   i
 Maurer, Martin H. [VerfasserIn]   i
Titel:Neurons and neuronal stem cells survive in glucose-free lactate and in high glucose cell culture medium during normoxia and anoxia
Verf.angabe:Sascha Wohnsland, Heinrich F. Bürgers, Wolfgang Kuschinsky, Martin H. Maurer
E-Jahr:2010
Jahr:03 July 2010
Umfang:8 S.
Fussnoten:Gesehen am 20.03.2024
Titel Quelle:Enthalten in: Neurochemical research
Ort Quelle:Dordrecht [u.a.] : Springer Science + Business Media B.V, 1976
Jahr Quelle:2010
Band/Heft Quelle:35(2010), 10, Seite 1635-1642
ISSN Quelle:1573-6903
Abstract:Several questions concerning the survival of isolated neurons and neuronal stem and progenitor cells (NPCs) have not been answered in the past: (1) If lactate is discussed as a major physiological substrate of neurons, do neurons and NPCs survive in a glucose-free lactate environment? (2) If elevated levels of glucose are detrimental to neuronal survival during ischemia, do high concentrations of glucose (up to 40 mmol/L) damage neurons and NPCs? (3) Which is the detrimental factor in oxygen glucose deprivation (OGD), lack of oxygen, lack of glucose, or the combination of both? Therefore, in the present study, we exposed rat cortical neurons and NPCs to different concentrations of d-glucose ranging from 0 to 40 mmol/L, or 10 and 20 mmol/L l-lactate under normoxic and anoxic conditions, as well as in OGD. After 24 h, we measured cellular viability by biochemical assays and automated cytochemical morphometry, pH values, bicarbonate, lactate and glucose concentrations in the cell culture media, and caspases activities. We found that (1) neurons and NPCs survived in a glucose-free lactate environment at least up to 24 h, (2) high glucose concentrations >5 mmol/L had no effect on cell viability, and (3) cell viability was reduced in normoxic glucose deprivation to 50% compared to 10 mmol/L glucose, whereas cell viability in OGD did not differ from that in anoxia with lactate which reduced cell viability to 30%. Total caspases activities were increased in the anoxic glucose groups only. Our data indicate that (1) neurons and NPCs can survive with lactate as exclusive metabolic substrate, (2) the viability of isolated neurons and NPCs is not impaired by high glucose concentrations during normoxia or anoxia, and (3) in OGD, low glucose concentrations, but not low oxygen levels are detrimental for neurons and NPCs.
DOI:doi:10.1007/s11064-010-0224-1
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext: https://doi.org/10.1007/s11064-010-0224-1
 DOI: https://doi.org/10.1007/s11064-010-0224-1
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:Hypoxia
 Lactate metabolism
 Neural progenitor cells
 Neural stem cells
 Neuron
 Rat brain
K10plus-PPN:1883931134
Verknüpfungen:→ Zeitschrift

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