Zyxin mediation of stretch-induced gene expression in human endothelial cells

• Verfasst von: Wójtowicz, Agnieszka [VerfasserIn]
• Verfasst von: Babu, Sahana Suresh [VerfasserIn]
• Verfasst von: Li, Li [VerfasserIn]
• Verfasst von: Gretz, Norbert [VerfasserIn]
• Verfasst von: Hecker, Markus [VerfasserIn]
• Verfasst von: Cattaruzza, Marco [VerfasserIn]
• Titel: Zyxin mediation of stretch-induced gene expression in human endothelial cells
• Verf.angabe: Agnieszka Wójtowicz, Sahana Suresh Babu, Li Li, Norbert Gretz, Markus Hecker, and Marco Cattaruzza
• E-Jahr: 2010
• Jahr: [October 1, 2010]
• Umfang: 5 S.
• Illustrationen: Illustrationen
• Fussnoten: Originally published 12 Aug 2010 ; Gesehen am 21.03.2024
• Titel Quelle: Enthalten in: Circulation research
• Ort Quelle: New York, NY : Assoc., 1953
• Jahr Quelle: 2010
• Band/Heft Quelle: 107(2010), 7 vom: Okt., Seite 898-902
• ISSN Quelle: 1524-4571
• DOI: doi:10.1161/CIRCRESAHA.110.227850
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: endothelial dysfunction
• Sach-SW: gene expression
• Sach-SW: hypertension
• Sach-SW: stretch
• Sach-SW: zyxin
• K10plus-PPN: 1884027628

Abstract

Rationale: - - Prolonged exposure to enhanced stretch, such as in hypertension, triggers endothelial dysfunction, a hallmark of pathological vascular remodeling processes. Despite its clinical relevance, little is known about stretch-induced gene expression in endothelial cells. - - Objective: - - Here, we have characterized a new stretch-inducible signaling pathway and the subsequent changes in endothelial gene expression in response to stretch. - - Methods and Results: - - Using human primary endothelial cells, we observed that the protein zyxin translocates from focal adhesions to the nucleus solely in response to stretch. There, it orchestrates complex changes in gene expression by interacting with a novel cis-acting element found in all zyxin-regulated genes analyzed so far. By way of DNA microarray pathway analyses, stretch-induced changes in endothelial cell gene expression were systematically explored, revealing that zyxin mainly regulates proinflammatory pathways. - - Conclusions: - - Stretch appears to be an important factor in the development of endothelial dysfunction with zyxin as a potential therapeutic target to interfere with these early changes in endothelial cell phenotype.