Ein neues Antikoagulans zur Probengewinnung für Thrombozytenfunktionstests

• Verfasst von: Wolf, Johanna [VerfasserIn]
• Verfasst von: Sigl, Martin [VerfasserIn]
• Verfasst von: Giannitsis, Evangelos [VerfasserIn]
• Verfasst von: Ivandic, Boris [VerfasserIn]
• Titel: Ein neues Antikoagulans zur Probengewinnung für Thrombozytenfunktionstests
• Paralleltitel: A new anticoagulant for blood sampling tubes for platelet function tests
• Verf.angabe: Johanna Wolf, Martin Sigl, Evangelos Giannitsis und Boris Ivandic
• E-Jahr: 2010
• Jahr: 7. Mai 2010
• Umfang: 5 S.
• Illustrationen: Illustrationen
• Fussnoten: Gesehen am 21.03.2024
• Schrift/Sprache: Artikel deutsch, mit englischer Zusammenfassung
• Titel Quelle: Enthalten in: Laboratoriumsmedizin
• Ort Quelle: Berlin [u.a.] : de Gruyter, 1977
• Jahr Quelle: 2010
• Band/Heft Quelle: 34(2010), 3, Seite 149-153
• ISSN Quelle: 1439-0477
• DOI: doi:10.1515/jlm.2010.026
• Datenträger: Online-Ressource
• Sprache: ger
• Sach-SW: anticoagulant
• Sach-SW: Antikoagulans
• Sach-SW: aspirin
• Sach-SW: Aspirin
• Sach-SW: clopidogrel
• Sach-SW: Clopidogrel
• Sach-SW: platelets
• Sach-SW: Thrombozyten
• K10plus-PPN: 1884036414

Abstract

Background: The difficult pre-analytical conditions of platelet function diagnostics discourage its widespread use, e.g., to detect resistance to acetylsalicylic acid (ASA) and/or clopidogrel. Platelet activation during blood drawing and sample preparation cannot be prevented by citrate anticoagulation. Here, we tested a new blood sampling tube containing the anticoagulant benzylsulfonyl-D-argininyl-prolyl-4-amidinobenzylamide (Thrombovette ® , Probe & go, Osburg, Germany). Methods: A total of 46 emergency patients taking ASA and clopidogrel as well as 20 healthy volunteers were included in the study. Blood was drawn into Thrombovette ® and citrate S-Monovette ® tubes (Sarstedt AG&Co, Nümbrecht, Germany) and transported to the lab via the pneumatic tube system. Whole blood impedance aggregometry (model 560Ca, Chrono-Log, Havertown, PA, USA) was performed using collagen and ADP as platelet agonist (final concentrations 1 mg/L and 5 μM, respectively). Results: Reference ranges and cut-off values for the determination of drug resistance were obtained from the healthy cohort. Positive predictive values were 94.6% and 94.1% for inhibition of aggregation by ASA, and 68.0% and 53.3% for inhibition by clopidogrel using Thrombovette ® and citrate S-Monovette ® , respectively. Negative predictive values were 82.8% and 75.0% for ASA resistance and 87.8% and 83.3% for clopidogrel resistance using Thrombovette ® and citrate S-Monovette ® , respectively. Conclusions: Thrombovette ® is a promising new system for blood sampling and could improve the validity of platelet function diagnostics.