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| Verfasst von: | Chaudhary, Roohi [VerfasserIn]  |
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| | Goodman, Laureen S. [VerfasserIn] |
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| | Wang, Sai [VerfasserIn] |
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| | Asimakopoulos, Anastasia [VerfasserIn] |
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| | Weiskirchen, Ralf [VerfasserIn] |
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| | Dooley, Steven [VerfasserIn] |
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| | Ehrlich, Marcelo [VerfasserIn] |
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| | Henis, Yoav I. [VerfasserIn] |
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| Titel: | Cholesterol modulates type I/II TGF-β receptor complexes and alters the balance between Smad and Akt signaling in hepatocytes |
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| Verf.angabe: | Roohi Chaudhary, Laureen S. Goodman, Sai Wang, Anastasia Asimakopoulos, Ralf Weiskirchen, Steven Dooley, Marcelo Ehrlich & Yoav I. Henis |
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| E-Jahr: | 2024 |
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| Jahr: | 02 January 2024 |
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| Umfang: | 16 S. |
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| Illustrationen: | Illustrationen |
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| Fussnoten: | Gesehen am 27.03.2024 |
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| Titel Quelle: | Enthalten in: Communications biology |
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| Ort Quelle: | London : Springer Nature, 2018 |
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| Jahr Quelle: | 2024 |
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| Band/Heft Quelle: | 7(2024), Artikel-ID 8, Seite 1-16 |
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| ISSN Quelle: | 2399-3642 |
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| Abstract: | Cholesterol mediates membrane compartmentalization, affecting signaling via differential distribution of receptors and signaling mediators. While excessive cholesterol and aberrant transforming growth factor-β (TGF-β) signaling characterize multiple liver diseases, their linkage to canonical vs. non-canonical TGF-β signaling remained unclear. Here, we subjected murine hepatocytes to cholesterol depletion (CD) or enrichment (CE), followed by biophysical studies on TGF-β receptor heterocomplex formation, and output to Smad2/3 vs. Akt pathways. Prior to ligand addition, raft-dependent preformed heteromeric receptor complexes were observed. Smad2/3 phosphorylation persisted following CD or CE. CD enhanced phospho-Akt (pAkt) formation by TGF-β or epidermal growth factor (EGF) at 5 min, while reducing it at later time points. Conversely, pAkt formation by TGF-β or EGF was inhibited by CE, suggesting a direct effect on the Akt pathway. The modulation of the balance between TGF-β signaling to Smad2/3 vs. pAkt (by TGF-β or EGF) has potential implications for hepatic diseases and malignancies. |
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| DOI: | doi:10.1038/s42003-023-05654-9 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
kostenfrei: Volltext: https://doi.org/10.1038/s42003-023-05654-9 |
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| | kostenfrei: Volltext: https://www.nature.com/articles/s42003-023-05654-9 |
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| | DOI: https://doi.org/10.1038/s42003-023-05654-9 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | Growth factor signalling |
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| | Membrane structure and assembly |
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| K10plus-PPN: | 188448977X |
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| Verknüpfungen: | → Zeitschrift |
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Cholesterol modulates type I/II TGF-β receptor complexes and alters the balance between Smad and Akt signaling in hepatocytes / Chaudhary, Roohi [VerfasserIn]; 02 January 2024 (Online-Ressource)
