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| Verfasst von: | Suzuki, Risa [VerfasserIn]  |
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| | Woo, Jia Zheng [VerfasserIn] |
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| | Thumberger, Thomas [VerfasserIn] |
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| | Hofmann, Gero [VerfasserIn] |
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| | Wittbrodt, Joachim [VerfasserIn] |
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| | Tavhelidse-Suck, Tinatini [VerfasserIn] |
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| Titel: | Characterizing medaka visual features using a high-throughput optomotor response assay |
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| Verf.angabe: | Risa Suzuki, Jia Zheng Woo, Thomas Thumberger, Gero Hofmann, Joachim Wittbrodt and Tinatini Tavhelidse-Suck |
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| E-Jahr: | 2024 |
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| Jahr: | March 31, 2024 |
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| Umfang: | 26 S. |
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| Illustrationen: | Illustrationen |
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| Fussnoten: | Gesehen am 02.04.2024 |
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| Titel Quelle: | Enthalten in: bioRxiv beta |
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| Ort Quelle: | Cold Spring Harbor : Cold Spring Harbor Laboratory, NY, 2013 |
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| Jahr Quelle: | 2024 |
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| Band/Heft Quelle: | (2024) vom: März, Artikel-ID 2024.03.28.587314, Seite 1-26 |
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| Abstract: | Medaka fish (Oryzias latipes) is a powerful model to study genetics underlying the developmental and functional traits of the vertebrate visual system. We established a simple and high-throughput optomotor response (OMR) assay utilizing medaka larvae to study visual functions including visual acuity and contrast sensitivity. Our assay presents multiple adjustable stripes in motion to individual fish in a linear arena. For that the OMR assay employs a tablet display and the Fish Stripes software to adjust speed, width, color, and contrast of the stripes. Our results demonstrated that optomotor responses were robustly induced by black and white stripes presented from below in the linear-pool-arena. We detected robust strain specific differences in the OMR when comparing long established medaka inbred strains. We observed an interesting training effect upon the initial exposure of larvae to thick stripes, which allowed them to better respond to narrower stripes. The OMR setup and protocol presented here provide an efficient tool for quantitative phenotype mapping, addressing visual acuity, trainability of cortical neurons, locomotor response, retinal regeneration and others. Our open-source setup presented here provides a crucial prerequisite for ultimately addressing the genetic basis of those processes. |
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| DOI: | doi:10.1101/2024.03.28.587314 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
kostenfrei: Volltext: https://doi.org/10.1101/2024.03.28.587314 |
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| | kostenfrei: Volltext: https://www.biorxiv.org/content/10.1101/2024.03.28.587314v1 |
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| | DOI: https://doi.org/10.1101/2024.03.28.587314 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| K10plus-PPN: | 1884690580 |
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| Verknüpfungen: | → Sammelwerk |
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Characterizing medaka visual features using a high-throughput optomotor response assay / Suzuki, Risa [VerfasserIn]; March 31, 2024 (Online-Ressource)
