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Verfasst von:Trendel, Jakob [VerfasserIn]   i
 Boileau, Etienne [VerfasserIn]   i
 Jochem, Marco [VerfasserIn]   i
 Dieterich, Christoph [VerfasserIn]   i
 Krijgsveld, Jeroen [VerfasserIn]   i
Titel:PEPseq quantifies transcriptome-wide changes in protein occupancy and reveals selective translational repression after translational stress
Verf.angabe:Jakob Trendel, Etienne Boileau, Marco Jochem, Christoph Dieterich and Jeroen Krijgsveld
E-Jahr:2023
Jahr:11 August 2023
Umfang:20 S.
Illustrationen:Illustrationen
Fussnoten:Online veröffentlicht: 3. Juli 2023 ; Gesehen am 04.04.2024
Titel Quelle:Enthalten in: Nucleic acids research
Ort Quelle:Oxford : Oxford Univ. Press, 1974
Jahr Quelle:2023
Band/Heft Quelle:51(2023), 14, Seite 1-20
ISSN Quelle:1362-4962
Abstract:Post-transcriptional gene regulation is accomplished by the interplay of the transcriptome with RNA-binding proteins, which occurs in a dynamic manner in response to altered cellular conditions. Recording the combined occupancy of all proteins binding to the transcriptome offers the opportunity to interrogate if a particular treatment leads to any interaction changes, pointing to sites in RNA that undergo post-transcriptional regulation. Here, we establish a method to monitor protein occupancy in a transcriptome-wide fashion by RNA sequencing. To this end, peptide-enhanced pull-down for RNA sequencing (or PEPseq) uses metabolic RNA labelling with 4-thiouridine (4SU) for light-induced protein-RNA crosslinking, and N-hydroxysuccinimide (NHS) chemistry to isolate protein-crosslinked RNA fragments across all long RNA biotypes. We use PEPseq to investigate changes in protein occupancy during the onset of arsenite-induced translational stress in human cells and reveal an increase of protein interactions in the coding region of a distinct set of mRNAs, including mRNAs coding for the majority of cytosolic ribosomal proteins. We use quantitative proteomics to demonstrate that translation of these mRNAs remains repressed during the initial hours of recovery after arsenite stress. Thus, we present PEPseq as a discovery platform for the unbiased investigation of post-transcriptional regulation.
DOI:doi:10.1093/nar/gkad557
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

kostenfrei: Volltext: https://doi.org/10.1093/nar/gkad557
 kostenfrei: Volltext: https://academic.oup.com/nar/article/51/14/e79/7217039
 DOI: https://doi.org/10.1093/nar/gkad557
Datenträger:Online-Ressource
Sprache:eng
K10plus-PPN:1885026714
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