SPIRO

• Verfasst von: Ohlsson, Jonas A. [VerfasserIn]
• Verfasst von: Leong, Jia Xuan [VerfasserIn]
• Verfasst von: Elander, Pernilla H. [VerfasserIn]
• Verfasst von: Ballhaus, Florentine [VerfasserIn]
• Verfasst von: Holla, Sanjana [VerfasserIn]
• Verfasst von: Dauphinee, Adrian N. [VerfasserIn]
• Verfasst von: Johansson, Johan [VerfasserIn]
• Verfasst von: Lommel, Mark [VerfasserIn]
• Verfasst von: Hofmann, Gero [VerfasserIn]
• Verfasst von: Betnér, Staffan [VerfasserIn]
• Verfasst von: Sandgren, Mats [VerfasserIn]
• Verfasst von: Schumacher, Karin [VerfasserIn]
• Verfasst von: Bozhkov, Peter V. [VerfasserIn]
• Verfasst von: Minina, Elena A. [VerfasserIn]
• Titel: SPIRO
• Titelzusatz: the automated Petri plate imaging platform designed by biologists, for biologists
• Verf.angabe: Jonas A. Ohlsson, Jia Xuan Leong, Pernilla H. Elander, Florentine Ballhaus, Sanjana Holla, Adrian N. Dauphinee, Johan Johansson, Mark Lommel, Gero Hofmann, Staffan Betnér, Mats Sandgren, Karin Schumacher, Peter V. Bozhkov and Elena A. Minina
• E-Jahr: 2024
• Jahr: April 2024
• Umfang: 17 S.
• Illustrationen: Illustrationen
• Fussnoten: Gesehen am 08.04.2024
• Titel Quelle: Enthalten in: The plant journal
• Ort Quelle: Oxford [u.a.] : Wiley-Blackwell, 1991
• Jahr Quelle: 2024
• Band/Heft Quelle: 118(2024), 2 vom: Apr., Seite 584-600
• ISSN Quelle: 1365-313X
• DOI: doi:10.1111/tpj.16587
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: 3D printing
• Sach-SW: Arabidopsis thaliana
• Sach-SW: automated image analysis
• Sach-SW: automated imaging
• Sach-SW: ImageJ macro
• Sach-SW: laboratory automation
• Sach-SW: open science hardware
• Sach-SW: phenotyping
• Sach-SW: plant autophagy
• Sach-SW: R
• Sach-SW: raspberry pi
• Sach-SW: root growth
• Sach-SW: seed dormancy
• Sach-SW: seed germination
• Sach-SW: technical advance
• Sach-SW: time-lapse imaging
• K10plus-PPN: 1885241429

Abstract

Phenotyping of model organisms grown on Petri plates is often carried out manually, despite the procedures being time-consuming and laborious. The main reason for this is the limited availability of automated phenotyping facilities, whereas constructing a custom automated solution can be a daunting task for biologists. Here, we describe SPIRO, the Smart Plate Imaging Robot, an automated platform that acquires time-lapse photographs of up to four vertically oriented Petri plates in a single experiment, corresponding to 192 seedlings for a typical root growth assay and up to 2500 seeds for a germination assay. SPIRO is catered specifically to biologists' needs, requiring no engineering or programming expertise for assembly and operation. Its small footprint is optimized for standard incubators, the inbuilt green LED enables imaging under dark conditions, and remote control provides access to the data without interfering with sample growth. SPIRO's excellent image quality is suitable for automated image processing, which we demonstrate on the example of seed germination and root growth assays. Furthermore, the robot can be easily customized for specific uses, as all information about SPIRO is released under open-source licenses. Importantly, uninterrupted imaging allows considerably more precise assessment of seed germination parameters and root growth rates compared with manual assays. Moreover, SPIRO enables previously technically challenging assays such as phenotyping in the dark. We illustrate the benefits of SPIRO in proof-of-concept experiments which yielded a novel insight on the interplay between autophagy, nitrogen sensing, and photoblastic response.