Hematopoietic activity of human short-term repopulating cells in mobilized peripheral blood cell transplants is restricted to the first 5 months after transplantation

• Verfasst von: Zavidij, Oksana [VerfasserIn]
• Verfasst von: Ball, Claudia R. [VerfasserIn]
• Verfasst von: Herbst, Friederike [VerfasserIn]
• Verfasst von: Fessler, Sylvia [VerfasserIn]
• Verfasst von: Schmidt, Manfred [VerfasserIn]
• Verfasst von: Kalle, Christof von [VerfasserIn]
• Verfasst von: Glimm, Hanno [VerfasserIn]
• Titel: Hematopoietic activity of human short-term repopulating cells in mobilized peripheral blood cell transplants is restricted to the first 5 months after transplantation
• Verf.angabe: Oksana Zavidij, Claudia R. Ball, Friederike Herbst, Sylvia Fessler, Manfred Schmidt, Christof von Kalle, and Hanno Glimm
• E-Jahr: 2010
• Jahr: June 17 2010
• Umfang: 3 S.
• Illustrationen: Diagramme
• Fussnoten: Gesehen am 17.04.2024
• Titel Quelle: Enthalten in: Blood
• Ort Quelle: Washington, DC : American Society of Hematology, 1946
• Jahr Quelle: 2010
• Band/Heft Quelle: 115(2010), 24 vom: Juni, Seite 5023-5025
• ISSN Quelle: 1528-0020
• DOI: doi:10.1182/blood-2010-02-271528
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1886032637

Abstract

Kinetics of hematopoietic recovery driven by different types of human stem and progenitor cells after transplantation are not fully understood. Short-term repopulating cells (STRCs) dominate early hematopoiesis after transplantation. STRCs are highly enriched in adult mobilized peripheral blood compared with cord blood, but the length of their contribution to hematopoiesis remains unclear. To understand posttransplantation durability and lineage contribution of STRCs, we compared repopulation kinetics of mobilized peripheral blood (high STRC content) with cord blood transplants (low STRC content) in long-lived NOD.Cg-PrkdcscidIl2rgtm1Wjl/SzJ (IL2RG−/−) mice. This comparison demonstrates that quantitative contribution of human STRCs to hematopoiesis is restricted to the first 5 months after transplantation. The ratio of STRCs to long-term repopulating cells dramatically changes during ontogeny. This model enables to precisely determine early and late engraftment kinetics of defined human repopulating cell types and to preclinically assess the engraftment kinetics of engineered stem cell transplants.