Expression of peptides encoded by exons in cloned mammalian DNA

• Verfasst von: Kreißig, Sabine [VerfasserIn]
• Verfasst von: Kutsche, Kerstin [VerfasserIn]
• Verfasst von: Dear, Neil [VerfasserIn]
• Verfasst von: Boehm, Thomas [VerfasserIn]
• Titel: Expression of peptides encoded by exons in cloned mammalian DNA
• Verf.angabe: Sabine Kreissig, Kerstin Schüddekopf, Neil Dear, Thomas BoehM
• E-Jahr: 1996
• Jahr: 01 November 1996
• Umfang: 2 S.
• Fussnoten: Gesehen am 23.04.2024
• Titel Quelle: Enthalten in: Nucleic acids research
• Ort Quelle: Oxford : Oxford Univ. Press, 1974
• Jahr Quelle: 1996
• Band/Heft Quelle: 24(1996), 21, Seite 4358-4359
• ISSN Quelle: 1362-4962
• DOI: doi:10.1093/nar/24.21.4358
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1886564175

Abstract

New synthetic approaches, such as combinatorial chemistry, provide a rich source of potential drug candidates. At the same time, the human genome initiative and other large-scale sequencing projects provide a large number of novel drug targets. However, the functional analysis of thousands of new genes remains a major challenge for the future. A systematic strategy for genome-wide functional analysis of genes could employ the fact that at least some modules in multi-domain proteins are encoded in individual exons. Exon amplification provides information about coding regions of most genes that is independent of their transcriptional status; exon amplification from entire mammalian genomes has been demonstrated. Here, we describe the development of an exon-trap system, λGEE (for genomic exon expression), that couples exon amplification with the expression of exon-encoded peptides.