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| Verfasst von: | Schäfer, Maximilian E. A. [VerfasserIn]  |
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| | Keller, Florian [VerfasserIn] |
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| | Schumacher, Jens [VerfasserIn] |
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| | Haas, Heinrich [VerfasserIn] |
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| | Vascotto, Fulvia [VerfasserIn] |
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| | Sahin, Ugur [VerfasserIn] |
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| | Hafner, Mathias [VerfasserIn] |
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| | Rudolf, Rüdiger [VerfasserIn] |
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| Titel: | 3D melanoma cocultures as improved models for nanoparticle-mediated delivery of RNA to tumors |
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| Verf.angabe: | Maximilian E.A. Schäfer, Florian Keller, Jens Schumacher, Heinrich Haas, Fulvia Vascotto, Ugur Sahin, Mathias Hafner and Rüdiger Rudolf |
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| E-Jahr: | 2022 |
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| Jahr: | 17 March 2022 |
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| Umfang: | 13 S. |
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| Fussnoten: | Gesehen am 30.04.2024 |
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| Titel Quelle: | Enthalten in: Cells |
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| Ort Quelle: | Basel : MDPI, 2012 |
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| Jahr Quelle: | 2022 |
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| Band/Heft Quelle: | 11(2022), 6, Artikel-ID 1026, Seite 1-13 |
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| ISSN Quelle: | 2073-4409 |
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| Abstract: | Cancer therapy is an emergent application for mRNA therapeutics. While in tumor immunotherapy, mRNA encoding for tumor-associated antigens is delivered to antigen-presenting cells in spleen and lymph nodes, other therapeutic options benefit from immediate delivery of mRNA nanomedicines directly to the tumor. However, tumor targeting of mRNA therapeutics is still a challenge, since, in addition to delivery of the cargo to the tumor, specifics of the targeted cell type as well as its interplay with the tumor microenvironment are crucial for successful intervention. This study investigated lipoplex nanoparticle-mediated mRNA delivery to spheroid cell culture models of melanoma. Insights into cell-type specific targeting, non-cell-autonomous effects, and penetration capacity in tumor and stroma cells of the mRNA lipoplex nanoparticles were obtained. It was shown that both coculture of different cell types as well as three-dimensional cell growth characteristics can modulate distribution and transfection efficiency of mRNA lipoplex formulations. The results demonstrate that three-dimensional coculture spheroids can provide a valuable surplus of information in comparison to adherent cells. Thus, they may represent in vitro models with enhanced predictivity for the in vivo activity of cancer nanotherapeutics. |
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| DOI: | doi:10.3390/cells11061026 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
kostenfrei: Volltext: https://doi.org/10.3390/cells11061026 |
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| | kostenfrei: Volltext: https://www.mdpi.com/2073-4409/11/6/1026 |
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| | DOI: https://doi.org/10.3390/cells11061026 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | cancer |
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| | in vitro in vivo correlation (IVIVC) |
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| | lipoplex |
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| | mRNA |
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| | nanoparticles |
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| | tumor models |
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| | tumor targeting |
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| K10plus-PPN: | 1887411666 |
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| Verknüpfungen: | → Zeitschrift |
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3D melanoma cocultures as improved models for nanoparticle-mediated delivery of RNA to tumors / Schäfer, Maximilian E. A. [VerfasserIn]; 17 March 2022 (Online-Ressource)
